Source: Thermo Fisher Scientific Inc.
Wednesday, October 28, 2009
ROCKFORD, Ill. (October 26, 2009) — Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific Pierce Co-Immunoprecipitation (Co-IP) Kit for the isolation of native protein complexes from a lysate or complex mixture using purified antibodies immobilized onto an agarose support. The kit uses an amine-reactive resin for the direct coupling of 10-50 micrograms of antibody. Traditional co-IP methods rely on Protein A or G and result in co-elution of the antibody heavy and light chains that may migrate with relevant bands, masking important results. The resin chemistry also enables immobilization that is independent of antibody isotype or species.
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The Pierce® Co-IP Kit includes optimized buffers for protein binding and recovery, reagents to perform control experiments and efficient spin columns and collection tubes that shorten the protocol and minimize handling. The optimized elution buffer provides a milder recovery of native protein complexes than the traditional boiling in SDS-PAGE, reducing sample buffer and making the isolated proteins more compatible with downstream applications. The protocol requires less sample and antibody.
The new Pierce Co-IP Kit supplies sufficient reagents to perform 50 co-IP reactions using 25 microliters of antibody support. The reagents can be stored at 4°C for up to one year.
For more information on the Pierce Co-IP Kit, please visit www.thermo.com/pierceor call +1-815-968-0747.
Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.
About Thermo Fisher Scientific
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With 2008 revenues of $10.5 billion, we have approximately 34,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. The Thermo Scientific brand represents a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike. Visit www.thermofisher.com.
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