Gene Walking Services

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APA genome walking

APA genome walking

Bio S&T

  • Catalog Number: BS170-01
  • Description: Bio S&T has developed a new method to isolate the transgene flanking sequences without reverting to a BAC library. This method is much more economical and the turnaround time is about 4 weeks. It is a modified walking method and is specifically tailored for multiple-copy transgenes. Pricing structure is as follows: Step #1 - $99 per walk Confirmation of known sequences. This step is non-refundable. Step #2 - $169 per direction Primer set will be designed and synthesized for 5’-direction and/or 3’direction walking. This step is non-refundable. Step#3 - $999 per library (per direction) Construction of a walking library. Should you need both the 5’ and 3’ flanking sequences, the second walking library will be offered at $599. This step is non-refundable. Step #4 - $899 per walk Cost will be $899 for sequencing data of one successful walking. A walk is considered successful when at least 150bp of flanking sequence is obtained beyond the border sequence. The length of each flanking sequence will vary from 150bp to 500bp. Each successful walk will be charged. Step #5 - $299/sequence We will design one primer based on the obtained sequence. In combination with one walking primer, we will use this primer pair to amplify a fragment of expected size. If unsuccessful, the obtained sequences will be regarded as false, and no charge for Step#4 will be applied. If transgene is present in multiple insertion locations, each successful confirmation will be charged (upon approval).
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