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Molecular Biology Videos
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Date Published
Title
Key Reagent in the SDS Lysis Buffer
February 13, 2023
We use UltraPure 10% SDS (sodium dodecyl sulfate) Solution when making the SDS lysis buffer, a buffer solution used for the purpose of breaking open ...
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Easy to Use Kit for Staining of Cell Nucleus
February 07, 2023
This dye is used for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.
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Using MagicMark™ XP Western Protein Standard with a Colometric Ladder is Next Level
February 02, 2023
When I started in my grad school lab, I was told that the previous graduate student mixed this ladder with a colorimetric ladder. This allowed him to ...
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Not My First Choice for coIP
January 25, 2023
I used these dynabeads to attempt coIP. However, I ran into several troubleshooting problems. The main issue I had was that the antibody I chose to IP...
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Reagent Works Great for Transfections
January 25, 2023
I used this to transfect my cells with a custom plasmid to produce an HA-tagged version of a protein. The reagents worked great. I was able to get ...
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First Recommendation for coIP
January 24, 2023
I used these beads to perform coIP in cell lysates. I like these magnetic beads because they do not use an antibody to capture your protein of ...
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Fixable Viability Dye for Dead Cell Staining
January 17, 2023
This viability dye irreversibly labels dead cells. I use it to exclude dead cells from FACS analysis.
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Fast and Easy Cloning for Sequencing
January 09, 2023
Cloning is really fast and easy with this kit, making sequencing very easy. PCR products amplified with Taq polymerase can be directly inserted into ...
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Cell Trace Violet to Quantify Proliferation
January 04, 2023
I work on immune responses between target cells such as HEK cells or B-EBV cells. To quantify this immune response, I use this product on PBMCs to see...
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Nice Calcein AM, Cell-Permeant Dye
December 21, 2022
We used this Calcein AM to stain the live cell. Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic ...
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Phospho-Kinesin 5B Antibody
December 19, 2022
We used this product to check the phosphorylation level of kinesin 5B protein. The antibody works well at the recommended dilution but gives some ...
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Useful Kit for Measuring Cell Proliferation using Flow Cytometry
December 12, 2022
This kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.
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Easy to Use Dye for the Detection of Cell Viability or Proliferation
November 23, 2022
The reagent is a high affinity, intracellular calcium indicator that is ratiometric and UV light—excitable that allows users to measure the cell ...
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MTT Cell Viability Assay
November 16, 2022
Is one of the simple assay to evaluate cell viability. It is a rapid colorimetric assay based on the cleavage of the tetrazolium ring of MTT (3-(4,5-...
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Nice DiR Near Infrared Dye Fluorescent Labeling Agent
November 14, 2022
Our research goal is to track the biodistribution of the exosome. We used DiR to stain the exosome as DiR is lipophilic and can incorporate into ...
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Strong Stain, Easy Optimization
November 04, 2022
Used to study the release of NETs from neutrophils in response to S. aureus stimulation in vitro. Great signal, enabling the identification of ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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