Protein purification can be a bit of an art, where even the smallest change to a protocol can have a big impact on the results.
HisTrap™ excel is equipped with a ligand to minimize the Ni-leakage and maximize protein binding.
So replace your conventional IMAC media with HisTrap excel columns for his-tagged proteins purification.
http://www.gelifesciences.com/histrapexcel
Visit our Protein Purification Information Base: http://www.proteins.gelifesciences.com