New Approaches To Multiplex QPCR Assays

Source : Integrated DNA Technologies

IDT invites delegates to technology workshops at Genomics Research Europe

CORALVILLE, IA – 29 August 2012 – Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, will be showcasing new approaches to multiplex qPCR assays which offer significant time and cost savings, at Genomics Research Europe, 4th-5th September 2012, Frankfurt, Germany, booth #14. Dr. Scott Rose, Director of Molecular Genetics at IDT, will be presenting a workshop on Multiplexing qPCR PrimeTime Assays with ZEN Dual-Quenched Probes (5th September 2012, 12:45pm – 1:30pm, AqPCR track room) followed by a Technology Spotlight on gBlocks™ Gene Fragments — Sequence-Verified PCR Fragments later that afternoon (3:15pm – 3:30pm).

In the workshop, Dr Rose will present data demonstrating how PrimeTime Predesigned qPCR Assays with ZEN double-quenched probes simplify a 4-plex assay system, saving time and money, and discuss factors that influence the success of using these assays in a multiplex format. The 15 minute technology spotlight will introduce IDT’s new multi-application molecular biology tool, gBlocks™ Gene Fragments. These double-stranded, sequence-verified DNA fragments up to 500 base pairs in length can be used as copy number standards for qPCR, without the need for cloning the endogenous amplicon. Multiple amplicons can be incorporated into a single gBlocks fragment, avoiding the need for linearizing plasmid controls and ensuring that concentrations of the standard dilutions are the same for the individual control assays in the multiplex reaction.

To register to attend Dr Rose’s workshop and learn more about the Technology Spotlight, visit the Genomics Research Europe website for further details. For more information on IDT’s qPCR technologies, please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the United States, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT's primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; and Leuven, Belgium. For more information visit www.idtdna.com.

Integrated DNA Technologies
800-328-2661 (US & Canada)
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www.idtdna.com

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