1 Doc In 5 Reports Being Stalked

Source : MedPage Today

PHILADELPHIA -- More than 20% of surveyed physicians said they had been stalked by a patient or former patient at some point, a researcher said here.Full Story
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  • Seeking a Happy (Cell Culture) Medium

    Seeking a Happy (Cell Culture) Medium

    Cell lines have been a staple of medical research at least since Henrietta Lacks’ ovarian tumor became HeLa cells more than half a century ago. Today, culturing continuous, immortalized cells is relatively routine—just take a bottle of medium off the shelf, throw in some serum (if it’s not already in there), add it to a flask and keep it cozy in a CO2 incubator. Primary cells and stem cells, on the other hand, are fussier. They have a limited life span, need a special blend of factors to grow and are susceptible to other factors that can take them down an undesired differentiation pathway. Here we look at some of the media researchers use to keep such finicky cells happy.
  • More than One Way to Change a Base

    More than One Way to Change a Base

    It’s easier than ever these days to clone and sequence DNA. Thanks to CRISPR/Cas and related technologies, it’s even straightforward to rewrite genomic sequences in living cells and organisms. But as powerful as it is, CRISPR, et al., cannot induce genetic rewrites in a test tube—genome editing requires cellular machinery to repair the DNA breaks the methods produce. Instead, researchers interested in mutating cloned genes on plasmids must revert to a tried-and-true method, site-directed mutagenesis. First described in the 1970s—and earning its inventor a share of the Nobel Prize in Chemistry in 1993—site-directed mutagenesis uses short oligonucleotides to introduce single base changes, as well as insertions and deletions, to DNA plasmids. Researchers can use the method to swap amino acids in expressed proteins, test clinically relevant mutations and tweak promoters. But there’s more than one way to change a base, and molecular-tools vendors have commercialized multiple strategies. Here, we review some of the more popular approaches to site-directed mutagenesis.

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