Edited by Barry S. SelinskyA collection of key techniques for the study of receptors and transport proteins. This book provides examples of how different membrane proteins can be over-expressed in both prokaryotic and eukaryotic expression systems, how natural and over-expressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
Edited by So IwataPart I: IntroductionPart II: Principles and Techniques in Membrane Protein CrystallizationPart III: Examples of Successful Crystallization of Membrane ProteinsPart IV: Crystallization Informatics of Membrane Proteins
by Petsko and RingeThis text introduces general principles of protein structure, folding, and function, then goes beyond these basics to tackle the conceptual basis of inferring structure and function from genomic sequence. Written for upper-level undergraduates and beginning graduate students, Protein Structure and Function will also be useful for working scientists needing an up-to-date introduction to the field.
by Alan FershtThere is a new era of protein design, sparked by the convergence of protein folding and enzymology. This book depicts the principles of protein structure, activity, and mechanism today. It takes a more general look at mechanisms in protein science, emphasizing the unity of concepts in folding and catalysis, as well as the importance of the relationships between basic chemistry, kinetics, thermodynamics, and structure. This book makes protein engineering easier to understand and apply.
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