Flow cytometry is a laser based technology used to count and sort cells, detect biomarkers and engineer proteins, by suspending cells in a stream of fluid and passing them by an electronic detection system. Flow cytometry is used in research and clinical laboratories for many applications including chromosome analysis, cancer diagnosis, protein expression, DNA and RNA quantification, multidrug resistance, and measuring enzyme activity. A system includes software, which should be user-friendly for flow cytometry novices, and advanced for experienced users to preform sophisticated experimental setups and analyses. Consider whether your laboratory needs a system that permits customization and provides imaging capability, and the degree of resolution your lab requires. Look for features such as automatic start-up, shutdown or cleaning, partially or fully automated processing, and quality customer support and service provided by the manufacturer.
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| Company | BD Biosciences | BD Biosciences | BD Biosciences | BD Biosciences |
| Item | BD FACSCalibur Flow Cytometer - 3-Color system with Sorting Option | BD FACSAria II Flow Cytometer: Blue and Red Laser Base Configuration | BD FACSAria II Flow Cytometer: Blue and Red Laser Base Configuration with ACDU | BD FACSAria II Flow Cytometer: Blue and Red Laser Base Configuration with ACDU and PMT Upgrade |
| Catalog Number | 342974 | 643177 | 643178 | 643179 |
| Price | | | | |
| Vendor Product Page | Company Product Page | Company Product Page | Company Product Page | Company Product Page |
| Description | The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system. Designed with multicolor application support in mind, the BD ... | The BD FACSAria II flow cytometer is a high-speed fixed-alignment benchtop cell sorter. The cytometer can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD ... | The BD FACSAria II flow cytometer is a high-speed fixed-alignment benchtop cell sorter. The cytometer can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD ... | The BD FACSAria II flow cytometer is a high-speed fixed-alignment benchtop cell sorter. The cytometer can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD ... |
| Absorbance / Excitation | Excitation Source- 488nm laser | Excitation- 488 nm, 633 nm and 405 nm Lasers | Excitation- 488 nm, 633 nm and 405 nm Lasers | Excitation- 488 nm, 633 nm and 405 nm Lasers |
| Detection | Up to 3 fluorescence and 2 scatter parameters | Octagon and trigon detector arrays | Octagon and trigon detector arrays | Octagon and trigon detector arrays |
| Voltage | Inquire | 100/115/230 VAC and 50 or 60 Hz | 100/115/230 VAC and 50 or 60 Hz | 100/115/230 VAC and 50 or 60 Hz |
| Volume | 10 to 200 µL | Inquire | Inquire | Inquire |
| Sample Type | 96 Well Plates
384 Well Plates | Inquire | Inquire | Inquire |
| Throughput | Inquire | 70,000 events per second with 8 parameters, 12 compensation pairs. Maximum rate ‹70,000 events per second with more parameters, ›100,000 events per second with fewer parameters. | 70,000 events per second with 8 parameters, 12 compensation pairs. Maximum rate ‹70,000 events per second with more parameters, ›100,000 events per second with fewer parameters. | 70,000 events per second with 8 parameters, 12 compensation pairs. Maximum rate ‹70,000 events per second with more parameters, ›100,000 events per second with fewer parameters. |
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