Blocking buffers and reagents are commonly used for immunodetection applications such as ELISA, Western blot, and immunohistochemistry. Blockers prevent the non-specific binding of antibodies, ensuring that the signal detected comes from the target analytes. Effective use of blocking buffers minimizes background noise as well as long-term preservation of samples. ELISA blocking buffers can help stabilize the plates for drying and storage, minimizing signal loss. Mixed reagents that help to reduce non-specific binding of primary antibodies and increase the storage time of the primary antibody working solution during Western blots are also available. Common reagents used in protein blocking include milk, casein, serum, and BSA. In nucleic acid hybridization and blotting applications, genomic DNA from calf thymus and herring or salmon sperm, have been used. Specialty reagents such as avidin/biotin blockers block all endogenous biotin and avidin binding activity. Browse the products below and visit the reagent supplier page for more information.
Your search returned 233 Blocking Buffers and Reagents across 35 suppliers.
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Sponsored Products
MyBioSource.com
- Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western ...
- 80 g
- ELISA (EIA), Western Blot (WB)
Bethyl Laboratories, Inc.
- 1 packet of dry powder to be dissoved in 1 liter H20
- 1 packet (makes 1 liter)
- ELISA
BPS Bioscience, Inc.
- This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience ...
- 50 ml
- Suitable for use with enzymatic assays to study enzyme ...
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Bio-Rad
- 30 ml
- Immunohistology - Paraffin,Immunohistology - Frozen
- Ready to use - liquid
- 500 ml
- ELISA
MilliporeSigma
- Used as a blocking agent in immunochemistry
- 100G, 500G
- mammalian cell culture
Rockland Immunochemicals, Inc.
- ELISA Microwell Blocking Buffer with Stabilizer is designed to block ELISA microwells coated with antigens, antibodies ...
- 1 L
- ELISA
- Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in ...
- 10 x 500 mL
- Western Blot
Biorbyt
- TRU BLOCK READY
- 20 ml
- Blocking, EIA, LF
- Blocking Buffer For Fluorescent Western Blotting
- 500 ml
- WB
Agrisera
- 2x100 ml (100 ml reagent A + 100 ml reagent B), enough for ...
- Immunoassay
- 2x1L (1L reagent A + 1L reagent B), enough for 100-200 midi ...
- Immunoassay
BioLegend
- Ce3D(TM) Permeabilization/Blocking Buffer
- 50 mL
- Immunohistochemistry (IHC)-F, 3D Immunohistochemistry (IHC)
- Ce3D(TM) Permeabilization/Blocking Buffer
- 15 mL
- Immunohistochemistry (IHC)-F, 3D Immunohistochemistry (IHC)
Candor Bioscience GmbH
- Total HAMA and interference blocker; sample diluent for reduction of interference caused by HAMA (human anti-mouse ...
- 500 mL
- ELISA, Multiplex Assay, Immuno-PCR
- Assay diluent for long-term storage of peroxidase conjugates and for minimizing cross-reactivities, matrix effects and ...
- 500 mL
- ELISA, Western Blot, Immunohistochemistry, Multiplex Assay
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MyBioSource.com
- Heterophilic Antibody (HA) interference in double-antibody sandwich reaction models can lead to false positive results ...
- 50 mg
- ELISA (EIA), Double-antibody immunoassays
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BPS Bioscience, Inc.
- 50 ml
- Suitable for use with enzymatic assays to study enzyme ...
- This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits
- 25 ml x 2
- Suitable for use with enzymatic assays to study enzyme ...
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Cell Signaling Technology
New England Biolabs
- SNAP-Cell Block™ (bromothenylpteridine, BTP) is a non-fluorescent compound that blocks the reactivity of the SNAP-...
- 100 nmol
- Cellular Biology
- SNAP-Surface Block™ (C8 propanoic acid benzylguanine, CBG) is a nonfluorescent compound that blocks the reactivity...
- 200 nmol
- Cellular Biology
LICORbio
- Try Intercept (PBS) Blocking Buffer and Intercept T20 (PBS) Antibody Diluent for improved results in your quantitative ...
- 1 kit
- Western blots, ICW, On-Cell Westerns
- Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for ...
- 10 x 500 mL
- Western blotting
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BioLegend
BioLegend
Bio-Rad
BioLegend
LICORbio
MilliporeSigma
BioLegend
Advansta