Peroxidase Substrates (DAB)

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DAB MEMBRANE PEROXIDASE SUBSTRATE

Rockland Immunochemicals, Inc.

Catalog Number: DAB-10
Description: Inquire
Quantity: 10 mL

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MaxTag DAB tablets (50 tablet pack)

Rockland Immunochemicals, Inc.

Catalog Number: DAB-50
Description: Inquire
Quantity: 1 Pack

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DAB CHROMOGEN CONCENTRATE

AbD Serotec

Catalog Number: BUF021A
Description: DAB CHROMOGEN CONCENTRATE
Quantity: 4 ml

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DAB CHROMOGEN CONCENTRATE

AbD Serotec

Catalog Number: BUF021B
Description: DAB CHROMOGEN CONCENTRATE
Quantity: 6 ml

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DAB

AnaSpec, EGT Group

Catalog Number: 85508
Description: Indicator for peroxidase activity; produce an insoluble brown product upon HRP/H2O2 oxidation
Quantity: 1 g

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Histofine DAB-3S kit

B-Bridge International

Catalog Number: 415194F
Description: A traditionally formated DAB substrate kit containing three components may not be familiar to some researchers. However this format provides enables more intense staining and that is stable for approximately 2 weeks.
Quantity: 1500 slides (153 ml)

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Histofine DAB-3S kit

B-Bridge International

Catalog Number: 415192F
Description: A traditionally formated DAB substrate kit containing three components may not be familiar to some researchers. However this format provides enables more intense staining and that is stable for approximately 2 weeks.
Quantity: 500 slides (51 ml)

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DAB Substrate Kit

BD Biosciences

Catalog Number: 550880
Description: Inquire
Quantity: 500 tests

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DAB Substrate

Bethyl Laboratories

Catalog Number: IHC-101F
Description: Inquire
Quantity: 250 slides

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DAB Tetrahydrochloride, Hydrate

Biomatik

Catalog Number: A2403-1g
Description: DAB Tetrahydrochloride, Hydrate
Grade: Reagent
Quantity: 1g

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DAB Tetrahydrochloride, Hydrate

Biomatik

Catalog Number: A2403-25g
Description: DAB Tetrahydrochloride, Hydrate
Grade: Reagent
Quantity: 25g

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DAB Tetrahydrochloride, Hydrate

Biomatik

Catalog Number: A2403-5g
Description: DAB Tetrahydrochloride, Hydrate
Grade: Reagent
Quantity: 5g

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Boost DAB Enhancer, 50x (SIGNET)

Covance, Inc.

Catalog Number: SIG-31047-3
Description: Covance?s DAB ENHANCER consists of a metal based solution that when used in conjunction with the DAB chromogen produces a clear brown - black precipitate that is insoluble in alcohol. The slides may be dehydrated through alcohols to xylene and mounted with any commercially available mounting media.
Quantity: 50x

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DAB 2 Component with Stabilizer (SIGNET)

Covance, Inc.

Catalog Number: SIG-31048-kit
Description:
Quantity: Inquire

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DAB Chromogen Concentrate (SIGNET)

Covance, Inc.

Catalog Number: SIG-31043-6
Description:
Quantity: Inquire

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DAB Chromogen Concentrate (SIGNET)

Covance, Inc.

Catalog Number: SIG-31041-4
Description:
Quantity: Inquire

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DAB Chromogen System, 1000 Test (SIGNET)

Covance, Inc.

Catalog Number: SIG-31042-kit
Description:
Quantity: 1,000 Test

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DAB Chromogen System, 300 Test (SIGNET)

Covance, Inc.

Catalog Number: SIG-31040-kit
Description:
Quantity: 300 Test

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DAB Substrate Buffer (SIGNET)

Covance, Inc.

Catalog Number: SIG-31045-100
Description: Substrate Buffer
Quantity: 2 x 50 mL

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AEC 50x Concentrate, Red, Horseradish Peroxidase Substrate (alcohol soluble)

EMD Millipore

Catalog Number: ES012-100ML
Description: AEC 50x Concentrate, Red, Horseradish Peroxidase Substrate (alcohol soluble)
Quantity: 100 mL

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AEC 50x Concentrate, Red, Horseradish Peroxidase Substrate (alcohol soluble)

EMD Millipore

Catalog Number: ES012-10ML
Description: AEC 50x Concentrate, Red, Horseradish Peroxidase Substrate (alcohol soluble)
Quantity: 10 mL

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DAB 50x Concentrate [3, 3'-diaminobenzidine], Horseradish Peroxidase Substrate (brown, insoluable in alcohol)

EMD Millipore

Catalog Number: ES005-100mL
Description: Since first introduced by Graham and Karnovsky (1), numerous procedures for its use in histochemistry, immunohistochemistry, Western and dot blots have been described (2-4). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (5). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins so often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB 1:50 with Chemicon Catalog Number ES010 (Tris-HCl buffer, pH 7.6), and adding H2O2, to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.
Quantity: 100 mL

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DAB 50x Concentrate [3, 3'-diaminobenzidine], Horseradish Peroxidase Substrate (brown, insoluable in alcohol)

EMD Millipore

Catalog Number: ES005-10mL
Description: Since first introduced by Graham and Karnovsky (1), numerous procedures for its use in histochemistry, immunohistochemistry, Western and dot blots have been described (2-4). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (5). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins so often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB 1:50 with Chemicon Catalog Number ES010 (Tris-HCl buffer, pH 7.6), and adding H2O2, to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.
Quantity: 10 mL

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DAB, Tetrahydrochloride

EMD Millipore

Catalog Number: 281751-10ML
Description:
3,3′-Diaminobenzidine
Dark brown solution. Produces a brown alcohol-insoluble end product. Also useful as a stain for myelin in glutaraldehyde-fixed sections. Supplied as a 50X concentrate. Buffer (Cat. No. 281753) is available as a 10X concentrate (pH 7.6 when diluted). Supplied with a directional insert. RTECS DV8753000, CAS 7411-49-6, M.W. 360.1.
Quantity: 10 ml

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DAB

GeneTex

Catalog Number: GTX30939
Description: DAB
Peroxidase chromogen-substrate 3-3´ diaminobezidine (DAB) reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives chocolate brown reddish color and is compatible with hematoxylin, nuclear fast red, methylene blue or Methyl green counterstain. This chromogen with above counterstains are insoluble in alcohol and organic solvents (dehydrating agents); therefore can be mounted with organic mounting mediums; e.g. our Organo mounting medium or others. DAB can also be mounted with aqueous mounting medium if hematoxylin is used as counter stain.
Quantity: 20 tablets

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DAB HRP Substrate - Chromogen ( 2 Liquid Components Kit )

GeneTex

Catalog Number: GTX73338
Description: This kit is supplied in 2 liquid components. 3A-DAB buffer 50ml 3B-DAB Chromogen 5ml 1. To 1 ml of 3A Buffer Reagent add one drop of 3B Chromogen Reagent Mix well. This ready to use reagent is good for 3-4 hours. 2. Wash specimen thoroughly with buffer (no azide in this buffer ) to remove traces of peroxidase. 3. Apply this solution to the specimen. 4. Incubate for 10-20 mins at RT or 37C. 5. Wash specimen with distilled or deionized water 5-7 times. 8. Counterstain. 9. DAB can be mounted with our Crystal mount ( We cat. # M02) an aqueous mountind medium or after dehydration with gradients of alcohol and toluene or xylene or any other organic dehydrating agent, followed by organic mounting medium
Quantity: 55 ml

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DAB with cobalt

GeneTex

Catalog Number: GTX30941
Description: DAB with cobalt
Peroxidase chromogen-substrate 3-3´ diaminobezidine (DAB) cobalt reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives dark black blue color and is compatible with nuclear fast red or Methyl green counterstains. This chromogen with these counterstains are insoluble in alcohol and organic solvents (dehydrating agents); therefore can be mounted with organic mounting mediums; e.g. Organo mounting medium or others.
Quantity: 20 tablets

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DAB with nickel

GeneTex

Catalog Number: GTX30940
Description: DAB with nickel
Peroxidase chromogen-substrate 3-3´ diaminobezidine (DAB) reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives chocolate brown reddish color and is compatible with hematoxylin, nuclear fast red, methylene blue or Methyl green counterstain. This chromogen with above counterstains are insoluble in alcohol and organic solvents (dehydrating agents); therefore can be mounted with organic mounting mediums; e.g. our Organo mounting medium or others. DAB can also be mounted with aqueous mounting medium if hematoxylin is used as counter stain.
Quantity: 20 tablets

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HRP chromogen kit ( DAB tablets )

GeneTex

Catalog Number: GTX28184
Description: The use on 3-3' dimaminobenzidine tetrahydrochloride (DAB) as a chromogen, when using peroxidase-based immunocytochemical procedures, provides the means of organic solvent stable permanent chromogen depositions. Therefore, specimens developed with DAB can be dehydrated and mounted with organic solvents-based plastic polymers such as Permount. Since DAB is a suspected carcinogen, many laboratories avoid the handling of the powdered chemical. However, the availability of DAB tablets minimizes the risk of exposure and facilitates its use in the immunohistology laboratory.

The DAB depositions are of a brown color and can be counterstained with alcohol?based hematoxylin or with Aqueous Hematoxylin.

4 plastic tubes:DAB tablets: 20 (plus one dissicant capsule.Concentrated Buffer: 5 mL. HRP detection substrate: 1 mL.Working reagent bottle: (empty). This kit contains sufficient reagents to make 100ml of final working solution
Quantity: 20 tablets

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DAB Immunohistochemistry Substrate

GenWay Biotech, Inc.

Catalog Number: GWB-55335F
Description: DAB Immunohistochemistry Substrate is supplied as a two component liquid substrate system designed for the rapid and simple detection of peroxidase in immunohistochemical and in situ hybridization procedures. The two components consist of DAB Immunohistochemistry Concentrate (Reagent A) and DAB Immunohistochemistry Reaction Buffer (Reagent B) which is a ready to use reaction buffer containing hydrogen peroxide.
DAB (3,3’-diaminobenzidine) is oxidized in the presence of peroxidase and hydrogen peroxide resulting in the deposition of a brown, alcohol-insoluble precipitate at the site of enzymatic activity. The precipitate may range in color from a light golden brown to dark brown depending upon the amount of enzyme present.
Quantity: 1.03 l

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DAB Reagent Set from KPL

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