Other Peroxidase Substrates

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ADHP

ADHP

AnaSpec, EGT Group

  • Catalog Number: 85500
  • Description: 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also called Amplex® Red and Ampliflu™ Red, is not only a sensitive and stable fluorogenic substrate for HRP but also an ultrasensitive probe for H2O2. In the presence of HRP and H2O2, ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, Zhou, et al. have demonstrated that ADHP can be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc. We offer the best quality of ADHP with the most competitive price. The reagent can be purchased in a single 25 mg vial or can be custom-packaged to meet your special requirements.
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HPPA

HPPA

AnaSpec, EGT Group

  • Catalog Number: 85505
  • Description: Fluorogenic peroxidase substrate
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10-Acetyl-3,7-dihydroxyphenoxazine

10-Acetyl-3,7-dihydroxyphenoxazine

Cayman Chemical

  • Catalog Number: 10010469
  • Description: 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.
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10-Acetyl-3,7-dihydroxyphenoxazine

10-Acetyl-3,7-dihydroxyphenoxazine

Cayman Chemical

  • Catalog Number: 10010469
  • Description: 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.
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10-Acetyl-3,7-dihydroxyphenoxazine

10-Acetyl-3,7-dihydroxyphenoxazine

Cayman Chemical

  • Catalog Number: 10010469
  • Description: 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.
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10-Acetyl-3,7-dihydroxyphenoxazine

10-Acetyl-3,7-dihydroxyphenoxazine

Cayman Chemical

  • Catalog Number: 10010469
  • Description: 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) is a highly sensitive, stable substrate for horseradish peroxidase (HRP) that enables selective detection of H2O2. This colorless, non-fluorescent reagent reacts with H2O2 to produce the fluorescent compound resorufin, which can be analyzed using an excitation wavelength of 520-550 nm and an emission wavelength of 585-595 nm. In a 96-well plate format, ADHP enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl sample.
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SuperSignal® HRP Substrate

SuperSignal® HRP Substrate

EMD Millipore

  • Catalog Number: 69059-3
  • Description: SuperSignal® HRP Substrate
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ELISA HRP Substrate (680) Pack

ELISA HRP Substrate (680) Pack

LI-COR Biosciences

  • Catalog Number: 926-34300
  • Description: The peroxidase (HRP) substrate results in a colorless to light blue product in the presence of increasing amounts of horseradish peroxidase. The reaction is stopped with a colored peroxidase stop solution, so the wells appear pink after the addition of stop solution.
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SuperSignal® HRP Substrate

SuperSignal® HRP Substrate

Merck

  • Catalog Number: 69059-3
  • Description: SuperSignal® HRP Substrate
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TACS Blue Label Substrate & Blue Streptavidin-HRP Diluent

TACS Blue Label Substrate & Blue Streptavidin-HRP Diluent

R&D Systems

  • Catalog Number: 4800-30-BL
  • Description: TACS Blue Label Substrate & Blue Streptavidin-HRP Diluent
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Ultra Blue™ Soluble

Ultra Blue™ Soluble

SeraCare Life Sciences, Inc.

  • Catalog Number: 92000-01K
  • Description: Ultra Blue Soluble is a Horseradish Peroxidase (HRP) liquid substrate, delivering enhanced signal intensity over TM Blue for quantified, enzyme immunoassays in microtiter plate, tube, or automated format. Stop solution: 2N sulfuric acid. Store at 2°-8°C. For research use only.
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CN/DAB Substrate Kit

CN/DAB Substrate Kit

Thermo Scientific Pierce Protein Research Products

  • Catalog Number: 34000
  • Description: A disadvantage of using DAB is that the resulting brown precipitate does not photograph well. The 4-Chloronaphthol substrate is not as sensitive as DAB, but photographs very well because of the low background development. Combining the two substrates yiel
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  • AnaSpec, EGT Group
  • Cayman Chemical
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  • LI-COR Biosciences
  • Merck
  • R&D Systems
  • SeraCare Life Sciences, Inc.
  • Thermo Scientific Pierce Protein Research Products