Simplified Homogenization Workflow to Maximize Protein Extraction and Minimize Variance Across Sample Types using Dry Pulverization and Adaptive Focused Acoustics
®
(AFA
®
) Technology
Meagan Gadzuk-Shea, Ph.D.
Friday, June 9, 2023
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
The Motivation and Goals for this Method Development
Outline
Dry Pulverization with Covaris Adaptive Focused Acoustics
®
(AFA
®
) for Tissue Sample Preparation
Experimental Design and Workflow
Workflow in Action
AFA
®
Parameter Definitions
Liver Tissue, Iteration 1: Establishing Baseline and Comparison to Bead Homogenization
An Important Note Before Proceeding
Extracted Protein Concentrations and Variance
AFA
®
Parameter Tuning
Testing Parameter Space: Liver Tissue
Application of Selected Parameters to Different Tissue Types of Varying Hardness
Revisiting Parameter Space: Kidney Tissue
Kidney and Brain Tissues
Testes, Pancreas, Intestine, and Liver Tissues
A Comparison of Hands-on Time: 24 Sample Prep
Revisit the Objectives of This Work
Key Take-aways and Next Steps
No transcript for this webinar
More Information
Request info