A Simplified High-throughput Cell-Based Assay for Increased Proteomic Coverage of Cardiomyocytes
Saeed Seyedmohammad, Ph.D.
Thursday, June 8, 2023
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Chapters
Introduction
A Simplified High-throughput Cell-Based Assay for Increased Proteomic Coverage of Cardiomyocytes
Background Research Context
Highlights of Previous Work
Assessing Cellular Proteome Coverage – I/R Cardiac Injury
Assessing Cellular Proteome Coverage in a Perturb System using a Fully Automated HTP Cell System – I/R Injury
Sonication During Digestion
To Optimize Protein Turnover in Cell Lysates – Experimental Design
Protein Quantification of Cell Lysates – Density Distribution
Protein Quantification of Cell Lysates – Principal Component Analysis
Protein Quantification of Cell Lysates - Proteome Dynamic Range
Differential Expression of Proteins - Volcano Plots and DEP Regulation
Differential Expression of Proteins – Violin Plots and Heatmap Analysis
Differential Expression of Proteins – Gene Ontology Annotations
Differential Expression of Proteins – Investigating Protein Load in Volcano Plot Analysis
Differential Expression of Proteins – Investigating Protein Load and Quantification
Sonication During Digestion – Missed Cleavage Analysis and Sub-Cellular Localization
Differential Expression of Proteins – Mitochondrial Network Interaction
To Further Optimize Protein Turnover in Cell Lysates – Unique Protein Detection
To Further Optimize Protein Turnover in Cell Lysates – Differential Expression of Protein Analysis
Future Directions
Summary Points
Thank You
Jenny Van Eyk Lab
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