Improved Adaptive Immune Receptor Repertoire Profiling for Biomarker Discovery
Alex Chenchik, PhD
Wednesday, May 24, 2023
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Chapters
Introduction
Gateway to Adaptive Immune System: TCR/BCR Repertoire Analysis & Phenotyping of Immune Cells
Generation of Diverse TCR and BCR Repertoire
Immunophenotyping of Adaptive T and B Cell Immune Responses
DriverMapTM Adaptive Immune Receptor (AIR) Profiling Assays
AIR-RNA Assay Outline
AIR-DNA Assay Outline
Comprehensive AIR-RNA Repertoire Analysis in Whole Blood and PBMC
TRB Clonotype Repertoire Analysis in Blood Samples
Reproducibility in TRB top 100 Clonotype Repertoire Analysis
TRB Repertoire Analysis in PBMC-Tissue mixes
Sensitivity of TCR/BCR Profiling: RNA vs DNA samples
AIR repertoire Analysis: Detection of Ag-activated clones
AIR assay: Detection of Neoantigen-Activated CDR3 Clones
T/B 500 Immune Marker Assay
AIR Immunophenotyping: T/B Cell Fractions vs Single-Cell Analysis
AIR Immunophenotyping in T/B Cell Fractions
AIR & Immunophenotyping for T Cell Sorted Fractions
Bulk Profiling vs AIR Immunophenotyping
Summary
Acknowledgments
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