Miniaturization of Metagenomic RNA-seq Library Prep
Eric Chow, PhD
Thursday, February 27, 2020
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Chapters
Introduction
Disclaimer
PLOS | ONE
Sequencing costs have dropped dramatically
Metagenomic sequencing
A few years ago, a diagnostic challenge
Phylogenic analysis matches timeline
Lower costs of NEB® kit lead to increased sample sizes
Formulatrix® Mantis™ Dispenser
TTP® Mosquito™
Echo 525 Liquid Handler
Other groups have miniaturized other library preps
Acoustic Droplet Ejection - ADE
Echo Technology Video
NEB® Ultra™ II RNA workflow
Minaturizing 10-fold
Minaturization requires lower input volumes
Integrity of dried RNA is intact
Lower elution volumes needed
384-well shim reduces elution volume
Minaturized libraries are good quality
Minaturized libraries are similar to full scale preps
Normalizing samples by sequencing
Normalizing output and inputs with Echo
rRNA removal
Qiagen® FastSelect™
Echo increases throughput and decreases cost
Full integration allows walkaway operations
Summary
Acknowledgements
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