Real-time Live-cell Analysis for Immunologists
Del Trezise, PhD
Friday, June 9, 2017
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Chapters
Introduction
Cells of the Immune System
Analytical methods in immunology
Current Limitations
Real-Time Live-Cell Analysis: IncuCyte®
Real-Time Live-Cell Analysis: Key Points
IncuCyte Key Feature: Cell Plates Never Move
IncuCyte Live-Cell Analysis workflow
Applications for immunology Researchers
Live-cell analysis: proliferation of suspension cells
Proliferation of WIL2NS B-cells: % Confluence Assay
Proliferation of PBMCs: % Confluence & Clustering
Combining live-cell analysis and flow cytometry workflows
Apoptotic cell death in suspesion cells
Time-course of apoptotic cell death: Jurkat T lymphocytes
Phagocytosis assays: IncuCyte pHrodo-E.Coli bioparticles
Differential phagocytosis of bacterial bio-particles by mouse macrophage populations
Viral infection analysis
Cytolysis assay: T-cell killing of human monocytes (THP-1)
T-cell killing in 3D tumor spheroids
CAR-T cell killing: proximity/attachment analysis
Chemotaxis/directiona; migration of immune cells
Chemotaxis of human M1 and M2 macrophages to C5a
Summary & Conclusions
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