Antibody Characterisation at MRC Technology
Rachel Forfar, PhD
Wednesday, March 1, 2017
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Chapters
Introduction
FORMING PARTNERSHIPS
COLLABORATION
IntelliCyt @ MRCT
IntelliCyt applications @ MRCT
Antibody-Drug-Conjugates
Antibody-Drug-Conjugate targets
1) Antibody binding
1) Cell binding: flow cytometry sec 1.
1) Cell binding: flow cytometry sec 2.
1) Cell binding: flow cytometry sec 3.
2) Screening hybridoma supernatants
ALK: Expi293 cells (overexpressed)
3) Antibody internalisation
3) Antibody internalisation: flow cytometry & imaging
Promega on-bead conjugation – pH dye (Promega)
3) Internalisation: flow cytometry
Acid wash method, strips surface binding
Promega on-bead conjugation – screening ALK hyb sups
3) Flow internalisation of A488 conjugates
IntelliCyt applications @ MRCT
Acknowledgements
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