Characterization of pediatric B- acute lymphoblastic leukemia using chromosomal microarray
Dr. Linda Baughn
Wednesday, August 3, 2016
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
Objectives
Pediatric B-ALL
Pediatric B-ALL: Improvements in survival
Risk stratification in B-ALL: the use of primary cytogenetic subtypes
Traditional practices for characterization of B-ALL
Limitations to G-banding | Limitations to FISH
Further identification of clinically relevant abnormalities still needed
Biology of B-ALL
Genetic components of B-ALL: Block in normal B-cell differentiation
Genetic components of B-ALL: Uncontrolled proliferation of blasts
Chromosomal microarray to fully characterize B-ALL
Advantages of SNP array
Limitations of SNP array
Why analyze B-ALL using SNP array?
How we analyze B-ALL using SNP array?
Example case 1: 7 year old male
Example case 1: Disease specific gene list
Microarray detection of iAMP21
Example case 1: 7 year old male | G-banding
Example case 1 : 1.3 Mb heterozygous deletion at 7p12.2
Example case 1: iAMP21
Examples 2 and 3: Aneuploidy
Example case 2: 12 year old female
Example case 2: 85% clonal fraction
Example case 2: Interpretation
Example case 2: 91% of low hypodiploid ALL with 32-39 chromosomes have TP53 mutations
Example case 3: 21 year old male
Example case 3: 50% clonal fraction
Example case 3: TP53 mutations identified in this patient
Example case 4: 3 year old female
Example case 4: Loss \ Gain
Conclusions
Acknowledgements
No transcript for this webinar
More Information
Request info
The views and opinions expressed by the guest speakers are solely their own and do not necessarily represent the views or opinions of Affymetrix, Inc.
Any Affymetrix product(s) referenced in this presentation are
For Research Use Only. Not for use in diagnostic procedures.
Please see
affymetrix.com/trademarks
for a complete list of Affymetrix trademarks. All other trademarks are the property of their respective owners.