Discovery of Antibody Drug Lead Candidates by Single Cell Function-Based Screening of Entire Antibody Secreting B-Cell Populations
Johan Jansson
Friday, March 18, 2016
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Chapters
Introduction
About HiFiBio - Company Summary
Raising the bar
Rapidly Access a Broad Diversity
CelliGOTM - Informed selection of multiple high quality lead candidates
Droplet Microfluidics
Modular Microfluidic Functions
A CelliGOTM case study Antibodies against tetanus toxoid
In droplet detection of antibody-antigen interaction
In-Drop Fluorescence Assay Design and Detection
Screening murine B cell repertoire for TT specific mAbs
A CelliGOTM case study Antibodies A1:F32 tetanus toxoid
Sorted B cell VH/VL paired sequences
Sorted B cell VH/VL paired sequences
Single cell-Hydrogel Bead co-Encapsulation
A fully integrated Bioinformatics workflow
Rapidly Access a Broad Diversity of Antibodies
A CelliGOTM case study Antibodies against tetanus toxoid
Dial-out PCR of antibody candidates: Implementation on the Echo picoliter dispenser
Rapid confirmation of anitbody activity
ECHO dispensing Step 1
ECHO dispensing step 2
Advantages of using ECHO
Rapidly Access a Broad Diversity of Antibodies
Additional assays we are currently developing
Detection of cell surface binders
Screening assay using live bacteria
In-droplet detection of antibody dependent activation of complement system
CelliGo impacts the entire preclinical development phase
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