TSA on LightCyclers - Collaborations
Rama Badugu, PhD
Monday, February 23, 2015
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Chapters
Introduction
Fluorescence based thermal Assays (TSA)
Requirements for an optimal Thermal shift run
LightCycler® 480 Real-time PCR System
Heart and soul of the instrument
Uniformity assay
Ligand titration (Lig dependant Tm Shift)
LightCycler® 1536 Real-Time PCR System
Strength Assessment of the Instrument
Linearity in Tm (lower range) over 8 degrees between LC1536 and LC480
Linearity in Tm (higher range) over 5 degrees between LC1536 and LC480
Summary
Acknowledgements
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