Enabling High Throughput qPCR
Lynn Rasmussen
Monday, February 23, 2015
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Chapters
Introduction
The Power of PCR
Automation of qPCR in 1536 Format
Liquid Handling Part 1
Pros and Cons
Dead Volume/Cost Comparison
How We Do It
Echo Dispenses Template
In a Biosafety Cabinet
PCR Destination Plate Enters Echo
Limitations
What do we Need to Amplify by PCR
Where Are We Applying qPCR?
Virus Titer Reduction
Limitation and Options
Purified RNA
Virus particles in media
Non Conventional Application
What is Needed for Success
After qPCR
Mirrorball-Laser Scanning Cytometer
Viral Protein on Live Cells
Influenza M2 Protein
Data Output
Disclaimer
Acknowledgments
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