1536 qPCR: A Robust Method for Detecting Changes in Gene Transcripts
Peter Aspesi
Monday, February 23, 2015
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Chapters
Introduction
Topics to Cover
Developmental and Molecular Pathways (DMP)
Intro to HTB
What HTB does
Robotics in HTB
Technology - Splice Variants
Technology - qPCR assay
Workflow – 1-step or 2-step RT-qPCR
2-Step
1-step process
One step vs two step
Reagents
Instrumentation
LightCycler® 1536 Real-Time PCR System: System Components - Instrument
LightCycler® 1536 Real-Time PCR System: System Components – Multiwell Plates
Workflow - 1536 qPCR
Challenges
50K Data Histogram
50K Data
Compound Collection
Follow Up Data
Conclusion
Acknowledgements
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