Metabolomics for Discovering the Functions of Genes with Embryonic-lethal Mutations
Steven S. Gross, PhD
Thursday, June 19, 2014
Tweet
Transcript
Embed
Email
Bookmark
<<
<
>
>>
Tweet
Facebook
Email
Chapters
Introduction
Phenotyping of Genetically-Engineered Mice
Htt Protein and the Disease
Function of Wildtype Htt Protein?
Untargeted Metabolite Profiling
Metabolite Profiling Workflow
MH & Profinder B06
Multivariate Chemometric Analysis, Metabolite ID, and Pathway Architect
Untargeted (-Ion) Metabolite Comparison of Htt Wildtype, KO and mutation in mESC
Metabolites (Neg Ions) with Significantly Altered Levels
Htt Gene Wildtype, Knockout and Mutation in Mouse ESCs
Release of Metabolites Into Culture Medium Informs on Metabolite Consumption and Release
Htt KO and Mutation on Expression on Glycolytic and Krebs Cycle
Unique Metabolites in Htt-/- mESC
Purine Biosynthesis and Salvage Pathways in mESCs
AMPK Signaling Promotes Glycolysis, Lipid Oxidation and Other Catabolic Pathways
Huntingtin KO in mESCs Results in Accumulation of Carnitine and Low MW Acyl-Carnitines
Huntingtin KO in mESCs
Huntingtin KO mESCs pt2
How Does Htt Impact Cell ATP Generation and Mt Bioenergetics?
Mt in Htt KO mESC are Defective in OXPHOS
Does Htt Deletion Impact Mitochondrial Structure?
Mt in Htt KO mESC are Structurally Aberrant
Does Htt Deletion Impact Mitochondrial Abundance, Mitochondrial Protein Content and Membrane Potential?
MitoTracker Red in Htt-/- Murine ES Cells vs. HttQ7/Q7 and HttQ140/Q7
Mitochondria-specific Protein Levels Aren’t Significantly Deceased In Htt KO mESC
JC1-Staining to Assess Htt gene Effect on Mitochondrial Membrane Potential
The Mitochondrial Respiratory Chain Drives Mitochondrial Membrane Potential
Summary and Conclusion
How to Explain Embryonic Lethality of htt KO?
Untargeted Metabolite Profiling
Multiomics: Integrating NGS RNASeq Data and Metabolomics
Acknowledgements
No transcript for this webinar
More Information
Request info