Simplicon™ RNA Reprogramming

Tuesday, July 1, 2014

Tweet

Transcript Embed

Email
Bookmark

<<
<
>
>>

Facebook

Email

Chapters

Introduction
Evolution of Reprogramming Technologies
Simplicon™ RNA Reprogramming
Advantages to Simplicon™ RNA Reprogramming Kit
Single co-transfection of VEE-OKS-iG and B18R RNAs
Kit Components
Before Starting: 2 Critical Parameters
Day 1
OKS-iG transgenes
Day 2
Day 4-11
Efficient reprogramming with Simplicon
PluriSTEM™
Painpoints in Human Pluripotent Culture
PluriSTEM™ Human ES/iPS Medium
Weekend-Free culture with PluriSTEM™
PluriSTEM™ is highly stable
Cells cultured in PluriSTEM™
Superior single cell culture with PluriSTEM™
Superior freeze-thaw viability with PluriSTEM™ Freezing Medium (SCM134)
Simple & easy transition to PluriSTEM™ (From either feeder-based or feeder-free systems)
PluriSTEM™ maintains cells in pluripotent state
PluriSTEM™: In vitro & in vivo differentiation potential
PluriSTEM™ Key Takeaways
PluriSTEM-XF™ Human ES/iPS Medium
Cells cultured in PluriSTEM-XF™ express appropriate pluripotent markers
Timecourse in PluriSTEM-XF™
Peripheral differentiation between colonies may occur in cell lines prone to differentiation
PluriSTEM-XF™ Key Takeaways
Enabling tools to allow researchers to create their model cell systems
Neural Induction from hESCs/iPSCs
Directed Neural Differentiation
Human ESC-derived Oligodendrocyte Progenitor Cells (SCR600)
Human ESC-derived OPCs are Able to Generate Mature Oligodendrocytes
In Vitro Myelination Assay
Posters & Technical Workshops
Acknowledgements

No transcript for this webinar

More Information Request info