Demonstration of Orthogonal Complementary Enrichment Methods for Enhanced Phosphopeptide Profiling of Drug-Treated Gastric Carcinoma Cells
Charles L. Farnsworth
Tuesday, June 24, 2014
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Introduction
Figure 1: Study Design
Figure 2: Motif Ab List
Figure 3: PTMScan Method
Figure 4: Control Western Blots
Figure 5: Motif Antibody Western Blots
Figure 6: Qualitative MS/MS Summary
Figure 7: Label-Free Quantitation
Figure 8: Relative Quantitation for SU11274 and Staurosporine Treated MKN-45 Cells
Figure 9: Relative Quantitation of Phosphorylation Sites on Met
Figure 10: Qualitative Comparison of IMAC and Motif Antibody Analaysis
Figure 11: Overlap of Motif Antibody Enrichment Strategies
Figure 12: Basophilic Motif Antibody Enrichment
Figure 13: KEGG Pathway Analysis
Conclusion
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