Two Dimensional LC-SRM Assay for Trastuzumab in Human Serum
Catalin Doneanu, PhD
Friday, January 24, 2014
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Chapters
Introduction
Workflow Overview
Methods
Protein to trypsin ratio optimization
Effect of incubation time on digestion efficiency
Reproducibility of trypsin digestion
Linearity of the 2D-SRM assay
RADAR monitoring of SPE-cleaned samples
Reproducibility of the 2D LC-SRM assay
Evaluation of signal suppression
Conclusions
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Application Note
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