Validation of an Automation Compatible High-Throughput Reverse Transcription qPCR Workflow
Catherine Bardelle, Ph.D.
Monday, February 3, 2014
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Chapters
Introduction
Current state and challenges for measuring transcriptional activity
Potential solution: the qPCR approach
qPCR principle: Taqman
Experimental implementation
Labcyte Echo Plate Reformat software and set up
Comparison of Echo VS manual dispensing
CT correlations
One step RT-qPCR in 384 and 1536w format
Dynamic range in 384w format
Dynamic range in 1536w format
Compounds potencies measured by qPCR
Scatter plot of pIC50 reporter gene assay VS qPCR
1536 challenges and next Steps
Summary
Acknowledgements
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