Size Exclusion Chromatography Basic Principles
Friday, December 27, 2013
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Chapters
Introduction
Size Exclusion Chromatography
Size Exclusion Chromatography Media Characteristics
SEC Column Liquid Volume Definitions
Visual display of Vo, Vt and Vt-V0 Column Volumes
Column Volume Definitions on a Chromatogram
Size Exclusion Chromatography Basic Run Conditions
Separation by Size Exclusion Chromatography in the Column
Separation by Size Exclusion Chromatography in the Column Over Time
Separation by Size Exclusion Chromatography: Elution Order
Separation by Size Exclusion Chromatography: Elution Order on a Chromatogram
Two Application Categories for Size Exclusion Chromatography
High Resolution Fractionation
High Resolution Fractionation Chromatogram Elution Profile
Method Development for high resolution fractionation
Group Separation
Factors Effecting Resolution
Sample Volume
SEC Column and Media Preparation & Efficiency
Media Selectivity & Separation Range
Selectivity Curves
Media Selectivity & Selection
Defining Fractionation Range & Exclusion Limit from a Selectivity Curve
Sample Preparation
Running Buffer Composition
Sample Application
Elution and Flow Rates
Care of Size Exclusion columns for separations
ENrich Size Exclusion Columns
ENrich SEC 650 Column - Great Performance!
Thank you!
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