Cell Line Cross-contamination: Impact on Biomedical Research
Yvonne Reid, PhD
Tuesday, March 26, 2013
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Chapters
Introduction
Outline
ATCC – Who We Are
Accreditations
ATCC is an Standards Development Organization
Henrietta Lack’s Cells Changed Modern Medicine
HeLa Cell Contribution to Biomedical Research
Poor Culture Conditions Lead to Contamination
Widespread Use of Cell Lines in Biomedical Research
Stanley Gartler Reveals HeLa Contamination
A standardized Collection of Animal Cell Lines
Karyotyping Reveals Interspecies Cross-contamination
Inter/Intraspecies Cross contamination: 1984-2003
Cellular Cross-contamination Persists: 2004-2010
Impact of Cellular Contamination on Research
Are Your Cells REALLY What You Think They Are?
Consequences of Cellular Contamination
Identification of Human Cell Lines
STR Analysis for Intraspecies ID of Human Cell Lines
Properties of STRs for DNA Profiling
Performing STR Analysis
Advantages of STR Analysis
STR Polymorphism
Human Cell Line Identification: STR Analysis
Human Cell Line Identification: STR Analysis (Cont.)
Case Study 1: Cellular Cross-contamination
Gender Is Important for Identification (AMELX Gene)
Case Study 2: Gender Misidentification
STR Analysis Used to Monitoring Genomic Stability
Case Study 3: Microsatellite Instability
STR DNA Profile Links Clone to Parent
STR Profile Analysis vs. Karyotyping
Case Study 4: Misidentified Cell Line
STR Testing Results
2007: Open Letter by Roland Nardone
Eradication of Contaminated Cell Lines: Call for Action
Consensus Standard for Cell Line Authentication
ANSI/ATCC: ASN-0002
Journals Are Requesting Authentication of Cell Lines
Steps for Reducing Cellular/Microbial Contamination
Steps for Reducing Cellular/Microbial Contamination
Services for STR Typing of Cell Lines
Thank You
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