Mastercycler® nexus Series Thermal Cyclers from Eppendorf

Product Specs
ItemMastercycler® nexus Series Thermal Cyclers
CompanyEppendorf
Price Get Pricing
Catalog NumberMastercycler nexus
Dimensions(WxDxH) 25 x 41.2 x 32.1 cm (9.8 x 16.2 x 12.6 inches)
Electrical Requirements120 V, 50/60 Hz
Temperature Range4 to 99 °C
Capacity96 x 0.2 mL PCR tubes, 1 PCR plate 8 x 12 or up to 71 x 0.5 mL PCR tubes
QuantityEA
MaterialBlock – Aluminum
TypePeltier elements, Triple Circuit Heating Technology
Wells96 Wells
Lid TypeHeated Lid
Heating RateApprox. 3 °C/s
Cooling RateApprox. 2 °C/s
DisplayBacklit display with keypad
ApplicationsPCR

Features

Temperature gradient; Connectivity (USB)

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Local Office

Eppendorf

100 Crossing Boulevard

Framingham, MA 01702

United States

Phone: 1-800-645-3050

Company Profile

Website: www.eppendorf.com

International Office

Eppendorf

Barkhausenweg 1

Hamburg 22339

Germany

Phone: +49 (0)1803 255 911

Email: eppendorf@eppendorf.de

Company Profile

Website: http://www.eppendorf.com/

(1) Genomic analyses reveal three phylogenetic species and their evolutionary histories in the big-headed turtleiScienceAugust 18, 2023Shiping Gong, Chatmongkon Suwannapoom, Minh Le, Truong Quang Nguyen, Yan Ge, Yufeng Wei, Yangchun GaoDalian, China), 10 pmol forward and reverse primers and 50 ng of genomic DNA. We performed PCRs on a Mastercycler nexus (Eppendorf, Hamburg, Germany) with the following cycle conditions: 95°C for 5 minutes; then 30 cycles

(2) Identification and anti-bacterial property of endophytic actinobacteria from Thymes kotschyanus, Allium hooshidaryae, and Cerasus microcarpaSci RepAugust 12, 2023Yaser Delbari, Yaser Mohassel, Elham Kakaei, Yadollah Bahramiuse. The isolates were subjected to 16S rDNA gene sequence analysis. The 16S rDNA was amplified using Mastercycler® nexus, Eppendorf. The 16s RNA gene of isolates was amplified using universal primers 27F (AGAGTTTGATCMTGGCTCAG)

(3) Herbicide resistance status impacts the profile of non-anthocyanin polyphenolics and some phytomedical properties of edible cornflower (Centaurea cyanus L.) flowersScientific ReportsJuly 17, 2023Urszula Gawlik-Dziki, Barbara Wrzesińska-Krupa, Renata Nowak, Wioletta Pietrzak, Joanna Zyprych-Walczak, Aleksandra Obrępalska-Stęplowska(Table S2), 200 ng of genomic DNA, and 1 U of Q5 High-Fidelity DNA Polymerase (NEB). PCR was done in a Mastercycler nexus (Eppendorf, Hamburg, Germany) with an initial denaturation at 98 °C for 30 s, followed by 35 cycles

(4) A retrospective evaluation of the Euroarray STI-11 multiplex system for the detection of eight STI causing agentsScientific ReportsJuly 14, 2023Karl Dichtl, Andreas Osterman, Johannes Forster, Lena Jakob, Sebastian Suerbaum, Michael J. Flaig, Sören Schubert, Johannes WagenerLabordiagnostika, Lübeck, Germany). Briefly, sample processing involved three steps: (1) multiplex PCR in a Mastercycler nexus SX1 block cycler (Eppendorf, Hamburg, Germany), (2) hybridization of the amplicons to a microarray chip

(5) In vitro Selection and in vivo Testing of Riboswitch-inspired AptamersBIO-PROTOCOLJuly 5, 2023Michael G. Mohsen, Ronald R. Breakerlamp (UVP, catalog number: UVG-65)Innova 42R shaking incubator (Eppendorf, catalog number: M1335-0004)Mastercycler Nexus GX2 (Eppendorf, catalog number: 6336000015)Milli-Q Advantage A10 (Millipore-Sigma, catalog number: Z00Q0V0WW)Mini

(6) Prevalence of Borrelia, Neoehrlichia mikurensis and Babesia in ticks collected from vegetation in eastern PolandExperimental and Applied AcarologyJune 30, 2023Anna Sawczyn-Domańska, Jacek Zwoliński, Anna Kloc, Angelina Wójcik-Fatlamaterial (Table S1). The PCR reactions were performed using C1000 Thermal Cycler (BioRad, USA) and Mastercycler Nexus (Eppendorf, Germany). DNA sequencing and sequence analysis Sequencing of PCR products was performed

(7) A Selection of 14 Tetrameric Microsatellite Markers for Genetic Investigations in Fallow Deer (Dama dama)Animals (Basel)June 23, 2023Orsolya Krisztina Zorkóczy, Orsolya Turi, Zsombor Wagenhoffer, László Ózsvári, Pál Lehotzky, Zsolt Pádár, Petra Zenkesize, and poor quantity or absence of product, the PCR settings were modified and optimized using a Mastercycler nexus GX2 (Eppendorf AG, Hamburg, Germany) gradient thermal cycler (Table S5). All PCR products resulting

(8) Chronicity of high and low level mupirocin resistance in Staphylococcus aureus from 30 Indian hospitalsScientific ReportsJune 22, 2023Rajni Prakash, Amar Garg, Riteshkumar Arya, R. K. KumawatµM) and primer mix (0.5 µL of 10pMol) and template DNA 1 µL were used in PCR cycle using Eppendorf Mastercycler® nexus PCR thermal cycler as the condition shown in Fig. 4. Control Staphylococcus aureus strain ATCC25923

(9) Dissecting the impact of dietary fiber type on atherosclerosis in mice colonized with different gut microbial communitiesnpj Biofilms and MicrobiomesJune 3, 2023Evan R. Hutchison, Kazuyuki Kasahara, Qijun Zhang, Eugenio I. Vivas, Tzu-Wen L. Cross, Federico E. Rey10 µL. A list of primers is shown in Supplementary Table 3. The cycling protocol was performed using Mastercycler® nexus (Eppendorf, Hamburg, Germany) as follows: 30 s at 95 ˚C, followed by 35 cycles of 10 s at 95 ˚C, 30

(10) FlipGFP protease assay for evaluating in vitro inhibitory activity against SARS-CoV-2 Mpro and PLproSTAR ProtocolsJune 1, 2023Haozhou Tan, Yanmei Hu, Jun WangChannels",Sartorius™,Cat#LH745421 HandE-Vac Handheld Aspirating System,Argos Technologies™,Cat#10-987-042 Mastercycler® nexus - PCR Thermal Cycler,Eppendorf,Cat#6331000025 "Reagent reservoir, sterile",VWR,Cat#89094-662 96-well

(11) Dietary disodium fumarate supplementation alleviates subacute ruminal acidosis (SARA)-induced liver damage by inhibiting pyroptosis via mitophagy-NLRP3 inflammasome pathway in lactating Hu sheepFrontiers in ImmunologyMay 19, 2023Hongzhu Zhang, Huimin Shi, Shendong Zhou, Guozhen Wei, Wan Xie, Meijuan Meng, Guangjun Chang, Xiangzhen Shenwere mixed, and adding nuclease-free water to a total of 10 μL. The reactions were carried out in a Mastercycler Nexus (Eppendorf). The cDNA was diluted with nuclease-free water for subsequent experiments. 10 μL of 2×ChanQ

(12) A short-term bioreactor assay to assess the effect of essential oils on a microbiota derived from piglet’s intestinal contentActa Veterinaria ScandinavicaMay 19, 2023Mathieu Bellerose, Philippe Fravalo, Isabelle Mainville, Yves Arcand, Alexandre Thibodeauanalysis of the microbiota. The V4 region of the genes coding for the 16S rRNA were first amplified in a Mastercycler ®Nexus (Eppendorf AG, Hamburg, Germany) with a total of 12.5 ng of genomic DNA from each sample and Platinum Superfi DNA Polymerase

(13) Prevalence of G6PD deficiency and diagnostic accuracy of a G6PD point-of-care test among a population at risk of malaria in MyanmarMalaria JournalMay 1, 2023Than Htike Aung, Chayanut Suansomjit, Zaw Min Tun, Tin Maung Hlaing, Jaranit Kaewkungwal, Liwang Cui, Jetsumon Sattabongkot, Wanlapa RoobsoongGoTagGreen Master Mix and 1 µl of 10 µM forward and reverse primers. Amplification was performed on a Mastercycler nexus GSX1 (Eppendorf), with first denaturation at 95 ℃ for 5 min, followed by the PCR cycling conditions

(14) Synchrospora gen. nov., a New Peronosporaceae Genus with Aerial Lifestyle from a Natural Cloud Forest in PanamaJournal of FungiApril 27, 2023Thomas Jung, Yilmaz Balci, Kirk D. Broders, Ivan Milenković, Josef Janoušek, Tomáš Kudláček, Biljana Đorđević, Marilia Horta Jungamplifications were performed using a LightCycler 480 II instrument (Roche, Basel, Switzerland) or Eppendorf Mastercycler nexus GSX1 (Eppendorf, Hamburg, Germany). All primers were synthesised by Elizabeth Pharmacon spol. s.r.o.

(15) Effects of Drought and Flooding on Phytohormones and Abscisic Acid Gene Expression in KiwifruitInternational Journal of Molecular SciencesApril 20, 2023Kirstin V. Wurms, Tony Reglinski, Poppy Buissink, Annette Ah Chee, Christina Fehlmann, Stella McDonald, Janine Cooney, Dwayne Jensen, Duncan Hedderley, Catherine McKenzie, et al.sample RNA and water to bring the final volume to 15 µL. Samples were hybridised in a thermocycler (Mastercycler® nexus, Eppendorf, Hamburg, Germany) at 67 °C for 19 h. They were then processed on a nCounter® Max platform

(16) Slight Temperature Deviation during a 56-Day Storage Period Does Not Affect the Microbiota of Fresh Vacuum-Packed Pork LoinsFoodsApril 19, 2023Charlotte Braley, Marie-Lou Gaucher, Philippe Fravalo, Fanie Shedleur-Bourguignon, Jessie Longpré, Alexandre Thibodeaufor 30 s, an elongation step at 72 °C for 60 s, and a final elongation step of 10 min at 72 °C in a Mastercycler®Nexus PCR (Eppendorf AG, Hamburg, Germany). Negative control of the DNA extraction (H20), as well as a PCR

(17) Molecular detection and assemblage analysis of the intestinal protozoan Giardia duodenalis in wild boars in KoreaFrontiers in Veterinary ScienceApril 18, 2023Haeseung Lee, Dongmi Kwakfirst-round PCR products, and 15 μL of sterile distilled water. PCR amplifications were performed using Mastercycler nexus (Eppendorf, Hamburg, Germany) with the following conditions: initial denaturation at 95°C for 5 min;

(18) A Global Survey of Hypervirulent Aeromonas hydrophila (vAh) Identified vAh Strains in the Lower Mekong River Basin and Diverse Opportunistic Pathogens from Farmed Fish and Other Environmental SourcesMicrobiology SpectrumApril 13, 2023Tingbi Xu, Cody R. Rasmussen-Ivey, Francesco S. Moen, Ana Fernández-Bravo, Brigitte Lamy, Roxana Beaz-Hidalgo, Chan Dara Khan, Graciela Castro Escarpulli, Ina Salwany M. Yasin, Maria J. Figueras, et al.increase PCR specificity (42), touchdown PCR was conducted to generate gyrB products and performed on a Mastercycler Nexus thermo cycler (Eppendorf, Hamburg, Germany) with 50 ng of genomic DNA (gDNA) isolated from each strain,

(19) Xiaoyaosan ameliorates depressive-like behavior and susceptibility to glucose intolerance in rat: involvement of LepR-STAT3/PI3K pathway in hypothalamic arcuate nucleusBMC complementary medicine and therapiesApril 12, 2023Qiu W, Wu Q, Zhang K, Da X, Tang K, Yuan N, Deng L, Wu M, Zhang Y, Quan J, et al.total RNA was reverse transcribed into cDNA using PrimeScript™ RT Master Mix (Takara, Cat. RR036A) on a Mastercycler® nexus gradien (Eppendorf, Germany). cDNA was amplified using the TB Green™ Advantage® qPCR Premix kit (Takara,

(20) Spinach (Spinacia oleracea) as green manure modifies the soil nutrients and microbiota structure for enhanced pepper productivityScientific ReportsMarch 13, 2023Ryeong-Hui Kim, Setu Bazie Tagele, Minsoo Jeong, Da-Ryung Jung, Dokyung Lee, TaeHyung Park, Bashizi Flory Tino, Kyeongmo Lim, Min A. Kim, Yeong-Jun Park, et al.were PCR-amplified with ITS86F/ITS4R59,60 and the universal primers 515F/907R61, using an Eppendorf Mastercycler® Nexus PCR Cycler (Eppendorf, Hamburg, Germany). The 50 µl PCR reaction mixture included 25 µl EmeraldAmp®

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Eppendorf Mastercycler Nexus