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Thursday, January 23, 2014
Proteomics can be bottom-up or top-down. The former is easier, the latter, more comprehensive -- especially when it comes to distinguishing one "...
Protein Biomarker Discovery Tools
Wednesday, November 27, 2013
While probing the genome certainly provides some leads as to the likelihood of disease development, it is protein biomarkers that speak to the here ...
Cell Fractionation Tools
Tuesday, November 26, 2013
Researchers fractionate cells for many reasons, from tracking protein translocation, to simplifying downstream analyses, to enhancing detection of low...
Post-Translational Modification Analysis
Thursday, September 19, 2013
In theory, sample purification for studying post-translational modifications isn’t really all that different from any other project. But you do need ...
A Little TLC For Your HPLC
Thursday, August 01, 2013
Keeping chromatography columns in tip-top condition will go a long way not only toward improving and standardizing your separations but also ensuring ...
The ups and downs of GC-MS
Thursday, June 06, 2013
GC-MS is the yin to LC-MS’s yang. Where LC-MS is solvent-based, GC-MS is a gas-phase technique. As a result, LC-MS is ideal for peptides, drugs, and ...
Lipidomics: A Decade of Growth
Tuesday, June 04, 2013
Though once regarded largely as cellular junk, material to be eliminated before getting to the more interesting proteins and nucleic acids, lipids are...
Conquering Cells to Extract the Proteins Within
Tuesday, May 28, 2013
When it comes to protein extraction, it’s all about balance: You need a method strong enough to crack open the cell wall and/or membrane, yet gentle ...
Protein Separation via PAGE for Multiple Applications
Tuesday, April 30, 2013
An overview of protein gel electrophoresis prior to Western blotting, mass spec, and other applications.
Metabolomics: Sifting Through Complex Samples
Thursday, March 14, 2013
Metabolomics seeks to catalog and quantify all the metabolites in a living organism. There are probably between 2,500 and 5,000 small molecules in the...
Mass Spectrometry and Liquid Chromatography: A Dynamic Duo
Thursday, January 24, 2013
Mass spectrometry (MS) enables researchers to investigate a host of properties, such as a sample’s molecular weight, structure, identity, quantity and...
Cancer Diagnostics – New Ways of Looking at Cancer Progression and Treatment
Tuesday, July 31, 2012
When cell signaling pathways go awry, uncontrolled growth of cancer cells may result. But there are so many types of cancer, in all kinds of cells, ...
Quantification of Complex Samples Using Multiple Reaction Monitoring
Tuesday, July 17, 2012
Quantification on a triple quad is generally accomplished via a tandem MS/MS assay known as selected reaction monitoring (SRM; also called single ...
ESI-TOF Mass Spectrometers
Tuesday, June 12, 2012
ESI requires no matrix. Instead, samples typically are injected into the instrument via either a syringe pump or liquid chromatography interface, such...
High-Resolution Mass Spectrometry
Tuesday, May 22, 2012
As its name implies, a high-res mass spectrometer is any instrument capable of resolving closely spaced spectral peaks, such that what appears to be, ...
CD169 (Siglec-1) Antibody: Works Out of the Box
The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters myeloid ...
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