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Software Tools for Gene Expression Analysis
Thursday, October 24, 2013
Gene-expression studies yield multitudes of data, and turning those data into biological insights requires software. A variety of options are ...
Choosing a qPCR Instrument
Thursday, September 26, 2013
qPCR is used for measuring RNA abundance, quantifying pathogen loads and validating microarray and sequencing data, among other applications. First, ...
RNA Prep from Problem Samples
Thursday, August 22, 2013
Expression analysis has become commonplace. First, though, you need to harvest the RNA. Here are tips for making the most of some difficult sample ...
Tools for Optimal RNA Prep: From Homogenization and Cell Lysis to Extraction and Purification
Wednesday, March 07, 2012
Extracting and purifying RNA from cells or tissues is no easy task, as anyone needing pure RNA for gene expression analysis will tell you. One of the ...
Absolute Quantification with Digital PCR
Wednesday, February 22, 2012
Digital PCR (dPCR) is a dream come true for anyone who has struggled with references and standards in making quantitative PCR (qPCR or real-time PCR) ...
Monday, May 02, 2011
If PubMed is any guide, the microRNA field is smokin' hot. Of the nearly 11,100 references that come up in a search for "microRNA," nearly two-thirds ...
Wednesday, April 06, 2011
Check the pages of most biology textbooks or research publications and you're sure to find at least one illustration of gene expression represented in...
Friday, February 20, 2009
PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. A conventional cloning procedure can ...
Excellent Antibody for T-Cell Activation
My research focuses on T cell based immunotherapy. For this, I regularly activate and expand primary T cells from PBMCs. Anti-CD3 antibody is a very potent tool...
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