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  • Differential Expression Analysis of RNA-Seq Data

    RNA-Seq and the Transcriptome

    Tuesday, May 07, 2013
    RNA-Seq builds on next-generation DNA sequencing (NGS), taking as its input RNA that has been reverse-transcribed into cDNA and (usually) PCR-... read more
  • Tools for Optimal RNA Prep: From Homogenization and Cell Lysis to Extraction and Purification

    Tools for Optimal RNA Prep: From Homogenization and Cell Lysis to Extraction and Purification

    Wednesday, March 07, 2012
    Extracting and purifying RNA from cells or tissues is no easy task, as anyone needing pure RNA for gene expression analysis will tell you. One of the ... read more
  • Absolute Quantification with Digital PCR

    Absolute Quantification with Digital PCR

    Wednesday, February 22, 2012
    Digital PCR (dPCR) is a dream come true for anyone who has struggled with references and standards in making quantitative PCR (qPCR or real-time PCR) ... read more
  • Master Mixes for Multiplex Real-time PCR

    Master Mixes for Multiplex Real-time PCR

    Wednesday, September 21, 2011
    The ability to run multiplex (also known as or quantitative PCR or qPCR) reactions is a challenging but important endeavor. Not only does it mean ... read more
  • Chromatin Immunoprecipitation (ChIP) Assays

    Chromatin Immunoprecipitation (ChIP) Assays

    Monday, August 29, 2011
    Suppose you identify a transcription factor that you believe regulates some gene of interest. It’s easy enough to demonstrate this protein is capable ... read more
  • Real-time PCR Systems

    Real-time PCR Systems

    Thursday, May 12, 2011
    There is a wide range of applications, including genotyping, SNP analysis, drug target validation, and quantitative gene expression analysis, which ... read more
  • microRNA Microarrays

    microRNA Microarrays

    Monday, May 02, 2011
    If PubMed is any guide, the microRNA field is smokin' hot. Of the nearly 11,100 references that come up in a search for "microRNA," nearly two-thirds ... read more
  • Epigenetics Tools

    Epigenetics Tools

    Wednesday, April 06, 2011
    Check the pages of most biology textbooks or research publications and you're sure to find at least one illustration of gene expression represented in... read more
  • Biomarker Discovery

    Biomarker Discovery

    Monday, March 01, 2010
    Imagine if you could peer into the proteome of your child and see raised warning flags denoting the future development of various diseases. Hopefully ... read more
  • DNA Microarrays

    DNA Microarrays

    Friday, May 22, 2009
    DNA microarrays are simply microscopic dots of DNA arrayed into a pattern, typically on a slide or chip, that facilitates analysis. Because small ... read more
  • PCR Cloning

    PCR Cloning

    Friday, February 20, 2009
    PCR cloning is a method of cloning which dramatically reduces the time and effort put into the cloning reaction. A conventional cloning procedure can ... read more