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DIY Antibodies for Western Blotting
Tuesday, March 04, 2014
The key to a good Western blot is the antibody, yet sometimes, the right antibody simply isn't available. In those cases, you'll need to either ...
Gel Documentation Software
Tuesday, December 03, 2013
Gel documentation systems automate the process of gel photography. Once the image is captured, additional software tools help users decipher the data....
Tips for Phosphospecific Antibodies
Thursday, October 10, 2013
Many researchers depend on the effectiveness and specificity of commercially available, phosphospecific antibodies. But with a wide range of ...
DIY Fluorescent Antibodies
Tuesday, September 24, 2013
Have a homemade antibody you want to label with a fluorophore? We break down some of the key variables to bear in mind as you get going.
Western Blot Troubleshooting
Thursday, July 11, 2013
In fact, some 41% of researchers say their Westerns fail at least a quarter of the time ... it’s not you; Westerns really are that finicky.
Protein Separation via PAGE for Multiple Applications
Tuesday, April 30, 2013
An overview of protein gel electrophoresis prior to Western blotting, mass spec, and other applications.
Western Blotting Continues to Evolve
Monday, February 04, 2013
Though Western blotting has for years been an important mainstay for protein researchers, many believe it has yet to reach its full potential. ...
Thursday, May 10, 2012
Most eukaryotic proteins are controlled by a series of chemical switches, or post-translational modifications. Glycosylation, the coupling of proteins...
Multiple Options for Faster Western Blotting
Thursday, March 01, 2012
Western blotting is a foundation technique for protein chemistry. The basic technique—transferring size-separated protein bands from a gel onto a ...
Cell Lysis Kits: Harvesting Your Starting Sample
Wednesday, April 27, 2011
Sometimes there is no better substitute for getting to your protein(s) of interest than simply breaking open the cell, heavy-handed though it may seem...
Western Transfer Systems: The Speed and Efficiency of Protein Blotting Increases
Monday, March 21, 2011
For decades, Western blotting (also known as protein blotting) has been instrumental in our ability to detect proteins separated by molecular weight ...
Cell Signaling Assays
Wednesday, November 17, 2010
Though often modeled in isolation, cells are social creatures. Like a teenager with unlimited texting, they send and receive a steady stream of ...
Fluorophores for Fluorescent Microscopy
Monday, October 18, 2010
Flip through any biological science journal today and chances are good you'll find at least one fluorescent micrograph. Painted in bright splashes of ...
Fluorescent Western Blotting
Monday, June 28, 2010
It's been nearly 30 years since W. Neal Burnette first coined the term "Western blotting" to describe the electrophoretic transfer of protein gels ...
The Western Blotting Workflow
Wednesday, February 10, 2010
Among molecular biologists and protein chemists, few techniques get as much play as the Western blot. Used to assess the presence, abundance, or ...
Autophagy: Finding the Line Between Normal and Diseased
Monday, February 08, 2010
Autophagy – literally, eating oneself ¬– is a crucial recycling function in cells whereby they rid themselves of intracellular matter such as protein ...
P53/ATM Aquarius Hematology FISH Probe
I am investigating chromosomal aberrations in lymphoma and have used the P53/ATM probe to assess gain and loss of these targets from fresh-frozen imprint slides...
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