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Picking a PCR Polymerase
Tuesday, October 15, 2013
Taq DNA polymerase works great for routine applications, but not every application is routine. Plus, there are those pesky replication errors. For ...
Optimizing PCR: Finding the Best Conditions for the Best Results
Monday, May 03, 2010
It really is science, right—not magic? Finding the best conditions for your PCR protocols can be frustrating and time-consuming, but it is becoming...
High-Tech Takes on Taq Polymerase
Friday, December 11, 2009
Taq DNA Polymerase, an enzyme that catalyzes the incorporation of dNTPs into DNA, is one of the most commonly used polymerases in molecular biology. ...
Friday, March 20, 2009
If PCR is a frequent activity in your laboratory, then a good way to save time is to make a master mix. A mix for PCR usually contains buffer, ...
Monday, March 09, 2009
Can you complete a PCR run in less than 30 minutes? If not, check out “fast PCR,” a term coined to refer to a standard PCR protocol that underwent a ...
Staining of CD82 in Human Peripheral Blood Neutrophils
Human peripheral blood neutrophils were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 30 minutes at 22°C. ...
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