PCR is a staple in any molecular biology lab, and a valuable tool in disciplines across the life sciences. PCR has become so commonplace that it’s easy to take for granted. But the wrong ratio of reagents in your reaction set-up, inefficient transfer of heat from block-to-tube-to-reaction, and evaporation during cycling are just some of the things that can throw off an otherwise perfectly good PCR. It’s a good idea to periodically evaluate your PCR reagents and instruments and make sure they are still serving your needs. Does your Taq enzyme offer the fidelity required by your application? Do your tubes and plates allow for even and efficient transfer of heat? Do they close/seal properly? Does your thermocycler still ramp to and maintain the desired temperatures? Do your plastics and thermocycler work together to minimize evaporation? As you consider these practical questions, peruse the products listed below. In addition to finding a product that addresses these fundamentals, you’re also likely to find products with additional features to make your PCR life simpler and more efficient.