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Date Published
Title
GenElute™ Gel Extraction Kit From Sigma
September 27, 2006
I use the GenElute™ Gel DNA Extraction Kit from Sigma when I want to carry out extraction of plasmid DNA from agarose gels. Most of my plasmid DNA ...
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REDExtract-N-Amp™ Tissue PCR Kit From Sigma
November 28, 2006
DNA extraction from tissues and subsequent PCR is something most biology labs do on a routine basis. Standard protocols call for DNA lysis at 55°C in...
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Sigma ProteoProfile Trypsin In-Gel Digest Kit
August 29, 2005
Extracting protein digests from SDS gels can be considered an art form, but with the right protocol in hand, the art can be handled more like a ...
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Genomeplex® Whole Genome Amplification (WGA) Kit by Sigma-Aldrich
January 31, 2007
The GenomePlex® Whole Genome Amplification method uses input DNA to generate a library of amplified DNA fragments that faithfully represents the ...
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ProteoPrep® Universal Extraction Kit From Sigma
February 08, 2007
Proteins located within the cytoplasm play an important role in signal transduction pathways and glycolysis. They also act as intracellular receptors ...
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GenElute® PCR Clean-up Kit From Sigma-Aldrich
February 02, 2007
The GenElute® PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) ...
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Biohit Proline Electronic Multichannel Pipettor From Sigma
January 02, 2007
The Biohit Proline 710800 is an 8-channel electronic repeat pipettor that is capable of pipetting 50-1200 ul of liquid at a given time. This ...
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ProteoPrep® Blue Albumin and IgG Depletion Kit From Sigma-Aldrich
February 14, 2007
Sigma’s ProteoPrep® Blue Albumin and IgG Depletion Medium contains a combination of 2 media: Blue dye conjugated to an agarose-based matrix and ...
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TRI Reagent® From Sigma-Aldrich
September 25, 2007
TRI Reagent® is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein. Successful isolations from human, animal...
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SIGMAFAST™ BCIP®/NBT B5655 Tablets From Sigma-Aldrich
May 13, 2009
The technique of Western blotting is a laboratory staple of protein expression analysis. After loading protein samples onto an appropriate gel and ...
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GenElute Plasmid Prep Kit From Sigma-Aldrich
September 29, 2009
In the modern molecular world almost every biology lab today finds itself working with plasmid DNA in some capacity. One of the simplest tasks ...
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REDExtract-N-Amp™ Tissue PCR Kit From Sigma-Aldrich
July 15, 2009
REDExtract-N-Amp™ Tissue PCR Kit is a good kit in my opinion. I am a graduate student. My research involves characterizing the role of two proteins ...
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Cell Growth Determination Kit, MTT Based From Sigma
June 19, 2008
The Cell Growth Determination Kit from Sigma is a colorimetric method for determining the number of viable cells in culture and is useful in the ...
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SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich
August 11, 2010
Quantitative real-time PCR (qPCR) is fast replacing conventional reverse transcriptase PCR in many molecular biology labs, increasingly reflected by ...
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Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting
February 14, 2012
In vivo
, we analyzed regenerating skeletal muscle, otherwise desmin expression was very low;
in vitro
desmin is normally expressed by myogenic cell ...
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Rabbit Anti-Pan Cadherin Antibody from Sigma-Aldrich, used in Immunofluorescence
January 17, 2012
I observed good staining in human colon epithelium cells and in smooth muscle cells (longitudinal and circular).
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Product Review
CD5 Antibody From BD Biosciences Has a Good Resolution
Ahmad Al
CD5, a very important antigen in T cell development, is expressed at high levels in mature T cells. It is also seen on B cells, either normally before they ...
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