Competent Cells (Yeast)

Competent Cells (Yeast)

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MaV203 Competent Yeast Cells, Library Scale
  • Catalog Number: 11281011
  • Description: MaV203 Competent Yeast Cells are designed for use with the ProQuest Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL 4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors (Figure 2). The MaV203 competent cells feature: Transformation (250 l of cells) efficiency yields 1 x 10 6 colonies Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants Competent cells formatted with carrier DNA already included
  • Quantity: 2 x 0.55 mL
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MaV203 Competent Yeast Cells, Subcloning Scale
  • Catalog Number: 11445012
  • Description: MaV203 Competent Yeast Cells are designed for use with the ProQuest Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL 4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest bait and prey vectors. MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors (Figure 2). The MaV203 competent cells feature: Transformation (250 l of cells) efficiency yields 1 x 10 6 colonies Control DNA pMAB12 to confirm transformation efficiency and the preparation of selection media Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants Competent cells formatted with carrier DNA already included
  • Quantity: 4 x 0.1 mL
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K. lactis GG799 Competent Cells

K. lactis GG799 Competent Cells

New England Biolabs

  • Catalog Number: C1001S
  • Description: Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC1-based expression vector.
  • Quantity: 5 rxns
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K. lactis Protease Deficient Competent Cell Sampler
  • Catalog Number: C1007S
  • Description: Chemically competent Kluyveromyces lactis cells are suitable hosts for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001S). Proteolysis of secreted heterologous proteins in K. lactis is most often casued by aspartyl poroteases that are present in the secretory pathway. In many cases, a single aspartyl protease causes the majority of the detrimental protein degredation. The Protease Deficient Competent Cell Sampler contains a wild-type GG799 strain, as well as four protease deficient K. lactis strains, each having selectable marker-free deletion of a specific secretory pathway aspartyl protease gene. Each strain can be transformed with any linearized pKLAC-series expression vector, permitting a comparison of heterologous proein quality and yield in each background.
  • Quantity: 5 rxns
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K. lactis YCT284 Competent Cells

K. lactis YCT284 Competent Cells

New England Biolabs

  • Catalog Number: C1002S
  • Description: Chemically competent Kluyveromyces lactis YCT284 (Δcts1) cells are a preferred host for secreting heterologous proteins carrying a chitin binding domain tag. YCT284 cells can be transformed with any linearized pKLAC-series expression vector. K. lactis strain YCT284 carries a selectable marker-free deletion of the KLLA0C04730g locus encoding an extracellular chitinase (KlCts1p) which can degrade chitin chromatography resin and compete for resin binding sites (1).
  • Quantity: 5 rxns
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  • Life Technologies
  • New England Biolabs