The chamber lower section is similar to the BSC-1 for temperature control and oxygenation. Pre-oxygenated medium enters the main body of the chamber through two separate lines. These are fine bore PTFE tubes which spiral in the heated distilled water in the lower part of the chamber and enter the upper part of the chamber. Here any bubbles in the line break and allow perfusion fluid to flow smoothly towards the slices. Acrylic templates allow the option for use of a single well or two separate wells. (Other designs can be supplied upon request). The templates are positioned on the chamber acrlylic base and silicone grease used to make the seal on the bottom (see diagram below). Typical dead space volume for the single well is approx. 200 ul and each of the wells in the dual version is approx. 100ul. The length of PTFE tubing carrying the perfusion solution to the wells has a volume of approx. 400ul. The flow rate of the perfusion fluid is adjusted at the exit well by means of lens tissue which is used to wick solution out of the exit port.
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