BAC Library Construction Services

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Arrayed BAC library

Bio S&T

Catalog Number: BS101-01
Description: Bio S&T has been constructing high-quality cDNA and DNA libraries since 1997. We have experience with bacterial, animal and plant genomes. We have also been successful in constructing BAC libraries from very challenging species such as marine bacteria or highly y GC-rich species. We pride ourselves in tailoring our services to suit our client’s needs. Our strengths: - We guarantee coverage. - High average insert and even insert size distribution. - We perform HMW DNA extraction at our facilities. - We have experience with plant, fungal, bacterial and animal libraries. - We have extensive experience with GC-rich organisms and marine bacteria. - Our arrayed BAC libraries are shipped in bar-coded microplates. - We have extensive experience shipping worldwide. - We provide technical support. - BAC libraries are the client's property and are never used internally, sold or distributed. - We provide references. Deliverables: - Genomic coverage is guaranteed. - Clones will be arrayed and amplified in 384-well plates (1 unique clone/well) and shipped on dry ice. - Plates will be identified with bar-coded labels. Related services: - Nylon filters: Available in small (8x12cm; maximum 3,072 clones) and large size (22.5x22.5cm; maximum 18,432 clones). Minimum order is 3 sets. The nylon filters will be ready for hybridization with the clones, in duplicate, cross-linked to them (Amersham Hybond-N+). - BAC library copy: Available for the arrayed BAC library option only. A copy of the Master BAC library can be made and stored or shipped. - Super pools and Pools derived from Arrayed BAC library: We can provide the following pools which will allow client to immediately begin screening: Plate Pools, Superpools, Row pools from Superpool plates, Column pools from Superpool plates.

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BAC Library Construction Services

Bio S&T

Catalog Number: BS101-01
Description: We offer arrayed BAC libraries, non-arrayed BAC libraries, screened BAC libraries (GeneScreen service) and filters. Our strengths: We guarantee coverage. We perform HMW DNA extraction at our facilities. We guarantee a minimum 100Kb average insert size (we usually obtain greater than 120kb) and, if desired, we can guarantee a greater minimum insert size (example: 150Kb or more). Our arrayed BAC libraries are shipped in bar-coded identified 384 well plates. We have extensive experience shipping worldwide and include a data logger to catalogue temperature during shipment. We provide technical support!

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Non-arrayed BAC library

Bio S&T

Catalog Number: BS103-01
Description: Bio S&T has been constructing high-quality cDNA and DNA libraries since 1997. We have experience with bacterial, animal and plant genomes. We have also been successful in constructing BAC libraries from very challenging species such as marine bacteria or highly y GC-rich species. We pride ourselves in tailoring our services to suit our client’s needs. Our strengths: - We guarantee coverage. - High average insert and even insert size distribution. - We perform HMW DNA extraction at our facilities. - We have experience with plant, fungal, bacterial and animal libraries. - We have extensive experience with GC-rich organisms and marine bacteria. - Our arrayed BAC libraries are shipped in bar-coded microplates. - We have extensive experience shipping worldwide. - We provide technical support. - BAC libraries are the client's property and are never used internally, sold or distributed. - We provide references. Deliverables: - Genomic coverage is guaranteed. - Client will receive BAC clone mixtures (transformed E. coli cells containing BAC DNA) stored in glycerol and shipped on dry ice. - An additional 5% of clones will be shipped at no extra charge to compensate for loss of titer during delivery. - This option is recommended to scientists studying large genomes and/or having arraying capabilities and/or wishing to limit shipping fees (no microplates to increase weight).

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Screened BAC library

Bio S&T

Catalog Number: BS104-01
Description: Bio S&T has been constructing high-quality cDNA and DNA libraries since 1997. We have experience with bacterial, animal and plant genomes. We have also been successful in constructing BAC libraries from very challenging species such as marine bacteria or highly y GC-rich species. We pride ourselves in tailoring our services to suit our client’s needs. A pooled BAC library is a very valuable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. Highlights: We typically construct a minimum 5X BAC library which provides a 99.3% probability of finding the target sequence. Additional coverage and 2-enzyme digestion is available (additional fee). BAC clones are then pooled and distributed in 96-well plates. For example, a BAC library created from a 3Gb species will consist of at least 125,000 BAC clones distributed into 3 x 96-well plates. Coverage is guaranteed. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Related services Screening: The pooled BAC library can be PCR-screened using client-provided PCR primer pair(s) which specifically amplifies a segment in the target area. Price per screening is dependent on genome size. Client can decided the number of positive BAC clones he wishes to purchase. Row and Column Pools derived from Pooled BAC library: We can also provide the following pools which will allow client to immediately begin screening. Advantages: The pooled BAC library can be screened hundreds of times and stored indefinitely. Our pooling method keeps the library in a compact format which is very easy to store. Copies can be easily made and economically shipped to collaborators worldwide. Drawback: The only disadvantage to this type of library is that it cannot be used for physical mapping or full-genome sequencing. Our strengths - We guarantee coverage. - High average insert and even insert size distribution. - We perform HMW DNA extraction at our facilities. - We have experience with plant, fungal, bacterial and animal libraries. - We have extensive experience with GC-rich organisms and marine bacteria. - Our arrayed BAC libraries are shipped in bar-coded microplates. - We have extensive experience shipping worldwide. - We provide technical support. - BAC libraries are the client's property and are never used internally, sold or distributed. - We provide references.

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Random Shear BAC Library Construction

Lucigen

Catalog Number:
Description: Lucigen is the recognized leader in Random Shear BAC library construction. We have created over 100 Random Shear BAC libraries from metagenomes, microbes, and plant and animal species for clients all over the world.

NO GAPS, even in centromeres!
• Unbiased BAC libraries without gaps.
• Larger inserts, fewer empty clones.
• Generate more results, faster, for less money.

Lucigen has developed technologies that overcome the problems associated with partial digestion library construction, producing much more even, complete, and higher quality genomic libraries.

• True, unbiased BAC libraries. Lucigen’s exclusive Random Shear Cloning Technology and CloneSmart® transcription-free vectors eliminate biases in DNA preparation and cloning. Clone gaps and sequencing gaps are practically eliminated.
• More BAC recombinants per nanogram of input DNA. Vector preparation methods, high efficiency CopyRight® v2.0 BAC vectors, and the 10G BAC-Optimized Replicator™ v2.0 Competent Cells substantially increase recombinant yields.
• Large BAC insert size. Random Shear BAC Libraries have a guaranteed average insert size of 100 kb or larger.
• Easy production of BAC DNA. A simple, single-step copy number induction in the CopyRight v2.0 system ensures high yields of BAC DNA, resulting in a >95% success rate in BAC end sequencing.
• Dramatically reduced finishing costs. Lucigen’s custom Random Shear BAC Libraries offer the greatest value in what counts…results obtained per dollar spent.

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Random Shear BAC Library Construction

Lucigen

Catalog Number:
Description: Lucigen is the recognized leader in Random Shear BAC library construction. We have created over 100 Random Shear BAC libraries from metagenomes, microbes, and plant and animal species for clients all over the world.

NO GAPS, even in centromeres!
• Unbiased BAC libraries without gaps.
• Larger inserts, fewer empty clones.
• Generate more results, faster, for less money.

Lucigen has developed technologies that overcome the problems associated with partial digestion library construction, producing much more even, complete, and higher quality genomic libraries.

• True, unbiased BAC libraries. Lucigen’s exclusive Random Shear Cloning Technology and CloneSmart® transcription-free vectors eliminate biases in DNA preparation and cloning. Clone gaps and sequencing gaps are practically eliminated.
• More BAC recombinants per nanogram of input DNA. Vector preparation methods, high efficiency CopyRight® v2.0 BAC vectors, and the 10G BAC-Optimized Replicator™ v2.0 Competent Cells substantially increase recombinant yields.
• Large BAC insert size. Random Shear BAC Libraries have a guaranteed average insert size of 100 kb or larger.
• Easy production of BAC DNA. A simple, single-step copy number induction in the CopyRight v2.0 system ensures high yields of BAC DNA, resulting in a >95% success rate in BAC end sequencing.
• Dramatically reduced finishing costs. Lucigen’s custom Random Shear BAC Libraries offer the greatest value in what counts…results obtained per dollar spent.

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Custom BAC Library Construction

Rx Biosciences

Catalog Number: RxBio-001
Description
: Rx Biosciences specializes in the construction and screening of BAC libraries. We have been associated with large-scale genome research projects such as genome sequencing, physical mapping, gene cloning and isolation of gene clusters for different metabolic pathways. The process of library construction involves the isolation of nuclei or protoplasts followed by preparation of agarose plugs. The nuclei or the protoplasts immobilized in agarose are digested with a restriction endonuclease and then fractionated on Pulse Field Gel Electrophoresis. Alternatively, we shear the genomic DNA by sonication followed by blunt ending and adapter ligation. The size fractionated genomic DNA is ligated to the BAC vector and electroporated into phage resistant competent cells. The clones are individually picked up and grown in 384 well plates.
Features
- Guaranteed genome coverage and insert size (125 to 150kb)
- Clones supplied in 96 well or 384 well plates in a special freezing medium that stabilizes the clones
- High density printing of BAC clones on nylon membranes in duplicate
- Pooling and super pooling of clones performed for easy library screening
- DNA isolated from pools and super pools are provided after screening
- Screening service offered
- Unbeatable time and price
- Isolation of BAC DNA and end sequencing offered

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Custom Random-Sheared BAC Library

Rx Biosciences

Catalog Number: RxBio - 001 Random Shear
Description
: Rx Biosciences is the ONLY SECOND Company in the world which offers construction of BAC libraries by random shearing approach. Our technology possesses several features that are superior to our competitor’s protocols allowing more efficient cloning of blunt ended genomic DNA. We use inducible pCC1 vector for preparing larger quantity of DNA from BAC clones for sequencing and, or restriction enzyme digestion.

Features:
- Preparation of organelle free pure nuclei (chloroplast/mitochondria contamination free)
- Precise monitoring of shearing of genomic DNA by viscometer
- Ligation of unique adapters at the end to enhances efficiency and clone insert size
- Higher ligation efficiency due to cohesive end ligation
- Maximum genome coverage with minimum or no gaps
- Cloning of restriction enzyme digestion resistant, AT-rich, GC-rich, un-clonable, and or large-insert DNA fragments including telomeres
- Save valuable time and cost in sequencing the genome or clone hunting
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Genomic Library In Lambda Phage Vector

Rx Biosciences

Catalog Number: RxBio-024
Description
: Rx Biosciences prepares genomic libraries in bacteriophage vector of customer's choice. We specialize in the construction of libraries from micro- quantities of the specimen such as trace amount of genomic DNA, chromosomes, uncultured environmental microbes, or base-modified phages. Highly methylated DNA, contamination with polysaccharides or phenolic compounds or restriction enzyme resistant DNA samples are successfully used to generate high quality libraries. The libraries are provided to the customer after rigorous Q.C. testing.
Features
- Vector of your choice such as lambda Dash II, EMBL 3, FIX II
- Average insert size from 15 to 23 kb.
- Guaranteed number of primary clones for complete genome coverage
- >95% recombinants
- Supplied as amplified or un-amplified library
- Quick turn around time
- Competitive pricing
- Library screening service is also provided