Confocal laser scanning microscopy (CLSM or LSCM) obtains high-resolution optical images by acquiring images point-by-point and reconstructing them with a computer, allowing three-dimensional reconstructions of topologically complex objects. These microscopes, found in biomedical clinical and research laboratories, are used in sensitivity demanding applications. There are two main subcategories- single point, often used in tissue slice imaging, and array scanning- often used for live cell imaging. The major optical microscopy vendors—Olympus, Nikon Instruments, Leica Microsystems and Carl Zeiss MicroImaging—offer confocal microscopes. When choosing a system, consider the applications such as live-cell imaging or fixed-tissue sections, FRET, multiphoton microscopy or super-resolution imaging. Expanding into different types of imaging requires upgradability. Also important in system selection is ease of use, availability of maintenance contracts and access to technical support.
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- Catalog Number:
- Speed: Varies with Model
- Resolution: 4 × 1 - 6144 × 6144 pixel
- Lasers: Varies with Model
- Detector: Varies with Model
- Catalog Number:
- Lasers: Varies with Model
- Detector: Varies with Model
- Catalog Number: TCS SP8
- Speed: Resonant scanner 8kHz: 28 - 290 fps
Resonant scanner 12kHz: 40- 428 fps - Resolution: Resonant scanner 8kHz: 1024 x 1024 pixel
Resonant scanner 12kHz: 832 x 832 pixel - Lasers: Solid State Laser / Diode Laser / Ar Laser / HeNe Laser / DPSS Laser / UV OPSL Laser
- Detector: PE APDs, MPD APDs, PTM Detectors
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Leica Microsystems (Microscopes)
- Catalog Number: TCS SMD FLIM
- Resolution: 8192 x 8192 pixel
- Lasers: Diode Laser / Ar Laser / HeNe Laser / DPSS Laser / UV Diode Laser
- Detector: Highly Sensitive Prism Spectral Detector / Transmitted Light Detector
Leica Microsystems (Microscopes)
- Catalog Number: TCS SPE
- Speed: 100 fps
- Lasers: Solid-state Laser
- Detector: Tunable Spectral Detector
Leica Microsystems (Microscopes)
- Catalog Number: TCS STED CW
- Speed: 25 fps
- Resolution: 8192 x 8192 pixel
- Lasers: Diode Laser / Ar Laser / HeNe Laser /DPSS Laser
- Detector: SP Detector
- Catalog Number: IMAGEXPRESS ULTRA
- Lasers: 488 nm and 635 nm solid state lasers
- Catalog Number: A1
- Speed: Non-resonant Scanner x2: 4 fps
Resonant Scanner: 30 - 230 fps - Resolution: Non-resonant Scanner x2: 4096 x 4096 pixel
Resonant Scanner: 512 x 512 pixel - Lasers: Ar Laser
- Detector: PMT Detector
- Catalog Number: A1R MP
- Speed: Standard Mode: 2 - 24 fps
Fast Mode: 10 - 130 fps
Scanning Speed: 30 - 420 fps - Resolution: Non-resonant Scanner x2: 4096 x 4096 pixel
Resonant Scanner: 512 x 512 pixel - Lasers: Laser Diode / Ar Laser / Solid-state Laser / G-HeNe Laser
- Detector: 4 PMT Detector
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- Catalog Number: A1R
- Speed: non-resonant scanner - 4 fps (512 x 512 pixels)
resonant scanner (X-axis),non-resonant scanner (Y-axis) - 30 fps (512 x 512 pixels) to 230 fps (512 x 64 pixels) - Resolution: 4096 x 4096 pixels / 512 x 512 pixels
- Detector: 4 PMT
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- Catalog Number: AZ-C1
- Speed: Standard: 1 fps
Bi-directional Scanning: 1.4 fps
Scanning Speed: 0.5 fps - Resolution: 160 x 160 - 2048 x 2048 pixel
- Lasers: Laser Diode / Ar Laser / Solid State / G-HeNe Laser/ Y-HeNe Laser / R-HeNe Laser
- Detector: Standars Flourcense Detector / Spectral Detector (C1si)
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- Catalog Number: SFC
- Speed: 120 fps
- Lasers: Air Cooled Argon Ion / Air Cooled Krypton-Argon / Water Cooled Krypton-Argon
- Detector: CCD Detector
- Catalog Number:
- Resolution: 64 x 64 - 4096 x 4096 pixel
- Lasers: LD Laser / Multiline Argon Laser
- Detector: Photomultiplier Detector
- Catalog Number: FluoView FV1000 Series
- Speed: 16 fps
- Resolution: 64 x 64 - 4096 x 4096 pixel
- Lasers: LD Laser / Multi Ar Laser / HeNe (G) Laser
- Detector: Varies with Model
- Catalog Number: FV1000MPE Basic
- Speed: 16 fps
- Resolution: 64 x 64 - 4096 x 4096 pixel
- Lasers: LD Laser / Multi Ar Laser / HeNe (G) Laser
- Detector: Reflected Light Fluorescent Detector / Transmitted Light Fluorescent Detector
- Catalog Number: FV1000MPE SIM
- Speed: 16 fps
- Resolution: 64 x 64 - 4096 x 4096 pixel
- Lasers: LD Laser / Multi-line Ar Laser / HeNe-G Laser
- Detector: Transmitted Fluorescence Light Detector
- Catalog Number: FV1000MPE TWIN
- Speed: 16 fps
- Resolution: 64 x 64 - 4096 x 4096 pixel
- Lasers: LD Laser / Multi Ar Laser / HeNe (G) Laser
- Detector: Reflected Light Fluorescent Detector / Transmitted Light Fluorescent Detector
- Catalog Number: FV10i-LIV / FV10i-DOC
- Speed: 1.1 fps
- Resolution: 256 x 256 - 1024 × 1024 pixel
- Lasers: LD Laser
- Detector: Photomultiplier
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