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Properties Of Reverse Transcriptases

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Properties of Reverse Transcriptases
Enzyme Maximum size of template Temperature optimum Units needed for standard reaction Ion require-
ment		 Rnase H activity Combined RT and DNA Pol. Incorporates DIG-11-dUTP
Expand Re-
verse Trans-
criptased		 up to 14kb 42°C (up to 50°C) 50 Mg2+ - - yes
AMV Reverse Transcriptase up to 12kb 42°C (up to 60°C) 40 Mg2+ ++ - yes
M-MuLV Reverse Transcriptase up to 10kb 37°C 40 Mg2+ + - yes
C. therm Polymerase for RT in Two-Step RT-PCR up to 4kb 60°-70°C 6 Mg2+ - -e yes
Tth DNA Polymerase up to 1kb 60°-70°C 4 Mn2+ - + yes
 
a Number in parentheses indicates maximum temperature tolerated by enzyme.
b Standard reaction performed as recommended in enzyme package insert.
c For nonradioactive labeling; also incorporates other modified nucleotides.
d Not for sale in the U.S.
e C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR does not survive incubation at 95°C. For RT-PCR, use the polymerase together with a second, thermostable enzyme such as Taq DNA Polymerase or Expand High Fidelity PCR System.

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Roche
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9115 Hague Road
Indianapolis, IN 46250-0414 USA




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