PCR Grade Deoxynucleotides

Highly purified deoxynucleotides for optimal results and maximum sensitivity in all PCR and RT-PCR applications

Unsurpassed purity and performance with PCR Grade Nucleotides!
Produce sensitive and consistent results with the newly developed PCR grade deoxy-nucleotides from RAS. Improve performance and sensitivity in PCR and RT-PCR assays, even when analyzing low copy number targets.

Significantly enhance your PCR without additional cost!

Use PCR grade dNTPs in daily applications, including PCR, RT-PCR, cDNA synthesis, sequencing, DNA labeling, and mutagenesis reactions. Our new manufacturing processes ensure higher quality without increasing production costs. Therefore, you obtain enhanced results without increasing your experimental costs.

Experimental results

Improve yield and performance

Purified to the highest possible purity (> 99% dNTP, < 0,9% dNDP), PCR-Grade Deoxynucleotides offer a secure starting point for a successful amplification reaction. Resulting from the new manufacturing process, PCR-Grade Deoxynucleotides are virtually free of dNTPs with modified bases, tetra- and pyrophosphate contamination that often inhibit amplification reactions (Figure 1).

Figure 1: Comparison of HPLC chromatograms between RAS’s PCR-Grade Deoxynucleotides and supplier A´s deoxynucleotides

–– Roche Applied Science –– Supplier A

Increase sensitivity

Assuring the highest possible functionality, PCR-Grade Deoxynucleotides are function-tested in a RT-PCR assay, the most sensitive and demanding amplification assay. Secured by this QC procedure, PCR-Grade Deoxynucleotides enable the detection of very rare messages (Figure 2).

Figure 2: Comparison of sensitivity between RAS's PCR-Grade Deoxynucleotides and supplier A's deoxynucleotides

Panel A shows that Roche’s PCR grade nucleotides produced an RT-PCR product from just 10 pg of human skeletal muscle total RNA (upper panel). It took at least 100 pg of RNA to produce similar results using Supplier A’s nucleotides (lower panel). Each lot of PCR grade nucleotides is assayed for function in an RT-PCR amplification of a 1849 bp fragment of the human dystrophin gene. Varying amounts (5000, 1000, 500, 250, 100, 50, and 10 pg) of human skeletal muscle total RNA are incubated with 1 ml Titan RT-PCR System enzyme mix. After reverse transcription and 35 cycles of PCR, the reaction is analyzed on a 1.0% agarose gel. To pass the quality control test, a PCR product must be produced from < 100 pg of total RNA.

Save money

PCR-Grade Deoxynucleotides are supplied as sodium salts in highly purified water at pH 8.3 without the addition of additives or stabilizers. Since most amplification reactions are performed between pH 8 and pH 9, PCR-Grade Deoxynucleotides are – compared to deoxynucleotides stored at pH 7 or 7,5 – more resistant to chemical stress caused by pH shifts (Figure 3). Additionally, this optimized buffer system guarantees stability for 24 months when stored at – 20 °C.

Figure 3: Stability comparison of deoxynucleotide solutions at varying pH

100 mmol/l Na⁺ solutions were stressed and the purity checked by HPLC. Storage at pH 8.3 significantly improves stability compared to storage at pH 7.5.

Product and Ordering Information: Individual dNTPs
Product		Cat. No.	Pack Size	Description	Application	Key advantages
Deoxy- nucleotides, PCR Grade	dATP	1934511 1969013	25µMol 125µMol	Single deoxynucle- otides, purified by HPLC; ready-to- use solutions, sodium salts, 100 mM	Optimal results and maximum sensitivity in all PCR and RT- PCR applications	Exceptional purity [dNTP, >99%; dNDP, <0.9% (HPLC, area %)] Tested for function in RT-PCR to ensure optimal performance Free of RNase, DNase, and nicking activity Free of additives that could interfere with PCR or RT-PCR Supplied at an optimal pH (8.3) for improved reaction stability and longer shelf life (24 months at –20°C)
	dCTP	1934520 1969021	25µMol 125µMol
	dGTP	1934538 1969030	25µMol 125µMol
	dTTP	1934546 1969048	25µMol 125µMol
	dUTP	1934554 1969056	25µMol 125µMol

Product and Ordering Information: dNTP-Sets and Mixes
Product	Cat. No.	Pack size	Description	Application	Key advantages
dNTP Set, PCR Grade	1969064	4 x 25 µMol	Convenient, ready-to-use set of four PCR Grade deoxynucleotides (dATP, dCTP, dGTP, dTTP, 100 mM solutions, sodium salts), in separate vials	Optimal results and maximum sensitivity in all PCR and RT-PCR applications	Same as individual PCR Grade Deoxynucleotides Separate vials of each dNTP for maximum flexibility
PCR Nucleotide Mix	1581295 1814362	100 react. 1000 react.	Premixed solution of all four deoxynucleotides (10 mM each)	Nucleotide mix for all PCR and RT-PCR applications	Premixed, ready to add directly to reaction mixed Resistant to repeated freezing and thawing Tested for stability and performance in PCR
PCR Nucleotide Mix^PLUS	1888412	2 x 100µl	Premixed solution of dATP, dGTP, dCTP (10 mM each) and dUTP (30 mM)	Nucleotide mix for UNG-mediated decontamination of PCRs and RT-PCRs	Same as PCR Nucleotide Mix Helps eliminate carry-over contamination