Optimizing Electroporation Parameters

Optimizing electroporation parameters

• Field strength
• Pulse length
• Number of field pulses
• Adjustment of the electroporation buffer
• Influence of DNA/RNA
• Influence of temperature
• Influence of the cell density

If one pulse proves to be insufficient, two or more pulses may be used to achieve the desired result, with a 60-second interval between pulses to allow the cell membrane to regenerate. During multiple pulsing, the Multiporator automatically maintains this interval between each pulse. An intact and resealed cell membrane is a prerequisite for the build-up of the membrane potential, which is essential for electroporation. In addition, the cells rotate during this regeneration phase (Brownian movement), which virtually rules out the danger of further injury being caused to the same membrane area by a second pulse.