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Optimizing Electroporation Parameters

Optimizing electroporation parameters

Other important parameters for optimizing electroporation.

Number of Field Pulses
For most cell lines, electroporation is carried out with one pulse.

If one pulse proves to be insufficient, two or more pulses may be used to achieve the desired result, with a 60-second interval between pulses to allow the cell membrane to regenerate. During multiple pulsing, the Multiporator automatically maintains this interval between each pulse. An intact and resealed cell membrane is a prerequisite for the build-up of the membrane potential, which is essential for electroporation. In addition, the cells rotate during this regeneration phase (Brownian movement), which virtually rules out the danger of further injury being caused to the same membrane area by a second pulse.

Contact Information

In the United States:
Eppendorf North America, Inc.
102 Motor Parkway,
Hauppauge, NY 11788-5178
Tel: 800-645-3050
Fax: 516-334-7506
Web Site: http://www.eppendorfna.com/

Outside the United States:
Eppendorf AG
Barkhausenweg 1
22339 Hamburg
Germany

Customer Service: ++ 49 40 53 801-0

Fax Number: ++ 49 40 53 801-556

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