A cell density of 1 x 106 cells/ml is recommended for electroporation since the electrical field is still effective on the cells in this range. When high cell densities > 3 x 106 are used, homogeneous field conditions can no longer be guaranteed; i.e. the cells are no longer evenly exposed to the electrical field. This may lead to cell fusion and cause the transfection rate to decrease. However, using a cell density of < 1 x 106 cells/ml should have no negative effect on the transfection rate.
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