Neogen offers two revolutionary TMB substrates—ALERT® K-Blue Aqueous and ALERT® K-Blue MAX—that utilize a patent-pending indicator system to clearly indicate when both substrate and acid stop have been added to microwells. After adding acid, the substrate produces a color change from colorless to pink, thus reducing the chance of false negatives due to reagent addition errors. Neogen’s ALERT® substrates offer additional advantages including: lot-to-lot consistency, ready-to-use one bottle format, long-term stability and high sensitivity. The ALERT® technology is specifically designed for sandwich-based HRP immunoassays but is proven to work in all microwell peroxidase-based immunoassays.
TMB substrates
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