Site specific endonuclease GlaI recognizes and cleaves only 5mC DNA. This enzyme belongs to a new type of 5mC site-specific DNA endonuclease discovered and characterized at SibEnzyme. The enzyme recognizes DNA sequence 5’-GC↓GC-3’/3’-CG↓CG-5’ with two or more 5-methylcytosines and cleaves it producing blunt ends. GlaI doesn’t cleave unmethylated DNA or DNA with C4- or N6A-modifications. Due to its ability to cleave only cytosine-modified DNA, GlaI may find a practical application in genetic engineering experiments as well as in determination of DNA methylation status in epigenomics studies.
5mC-dependent site-specific DNA endonuclease GlaI
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