Suppressing Things With siRNA Vectors

For shRNAs – TransFectin, a cationic lipid, efficiently delivers shRNA-expression plasmids and cassettes to facilitate effective knockdown results. TransFectin works with a broad range of cells, including some difficult-to-transfect and primary cells, and produces high efficiency and transgene activity while minimizing damaging cytotoxic effects. For siRNAs - siLentFect is a low-toxic cationic lipid designed to deliver siRNA to a broad range of adherent and suspension cultured mammalian cells. It has a strong affinity for siRNA and effectively produces 90-99% silencing with as little as 100 pM siRNA.

BioVision's GeneBlocker pGB siRNA expression vectors are designed to provide efficient suppression of a variety of apoptosis genes in cultured mammalian cells and in vivo. BioVision currently offers siRNA vectors blocking genes such as: AIF, BAD, BAX, Bcl-2, Bid, Caspases, cIAP-1, cIAP-2 and Hsp90. Each siRNA vector mix consists of up to 4 siRNA plasmids that target to different positions of the coding region of targeted mRNA. The pGB vector has been optimized for suppressing expression of target genes using the human U6 promotor (a RNA polymerase III promotor) which generates large amounts of siRNA in mammalian cells. All it takes is a simple transfection and you can observe substantially reduced gene expression. Company Website