| Eppendorf Perfectprep Plasmid DNA Isolation Kits are designed to generate
high yields of highly pure plasmid DNA suitable for automated fluorescent
sequencing, transfection, PCR, and other extremely sensitive downstream
applications.
In order to compare yields between our kits and the leading competitor,
side-by-side extractions were performed on aliquots from single cultures
of various strains of E.coli containing different plasmids. The results
are shown in Table 1. Clearly, the Perfectprep kits significantly outperformed
the leading competitor's kit in terms of yield every time.
Table 1: Perfectprep Midi vs. Brand X
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| Restriction digests using EcoR1 from NEB were performed on 250 ng of the
pCH110 DNA samples tabulated above. Lane order is as follows:
1) Sample 1, PP-Midi, uncut; 2) Sample 1, PP-Midi, 1U EcoR1;
3) Sample 1, PP-Midi, 5U EcoR1; 4) Sample 2, PP-Midi, uncut;
5) Sample 2, PP-Midi, 1U EcoR1; 6) Sample 2, PP-Midi, 5U
EcoR1; 7) Sample 1, Brand X, uncut; 8) Sample 1, Brand X,
1U EcoR1; 9) Sample 1, Brand X, 5U EcoR1; 10) Sample 2,
Brand X, uncut; 11) Sample 2, Brand X, 1U EcoR1; 12) Sample
2, Brand X, 5U EcoR1; 13) Lambda HindIII DNA Marker
Recover more DNA in less time!
Conclusion
Perfectprep Plasmid isolation kits offer a convenient, time and cost-effective
method for isolating plasmid DNA. Because each lot is performance tested
and the isolated DNA evaluated for quantity and quality using spectrophotometric
assay, digestion with restriction enzymes, automated fluorescent DNA cycle
sequencing, and transfection of cultured cells, the researcher is guaranteed
excellent quality, RNA-free plasmid DNA comparable to DNA purified using
a CsCl gradient. Typical follow-up applications include manual and automated
fluorescent sequencing, transfection, cloning, in vitro transcription,
and even microinjection.
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