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Optimizing Electroporation Parameters

Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Pulse Length
In addition to the field strength, a crucial factor for successful transfection is the pulse length. It is primarily dependent on the diameter of the cell: the larger the cell, the longer the pulse necessary for permeation of the membrane. Empirically, the ideal pulse lengths for electroporation have proven to be 40–100 µs at room temperature and 15–40 µs at 4°C. To optimize the duration of the pulse, three different pulse lengths should be selected within the above-mentioned ranges.

Contact Information

In the United States:
Eppendorf North America, Inc.
102 Motor Parkway,
Hauppauge, NY 11788-5178
Tel: 800-645-3050
Fax: 516-334-7506
Web Site: http://www.eppendorfna.com/

Outside the United States:
Eppendorf AG
Barkhausenweg 1
22339 Hamburg
Germany

Customer Service: ++ 49 40 53 801-0

Fax Number: ++ 49 40 53 801-556

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