Four New CastAway™ Precast Gels
CastAway precast gels* eliminate the need to manually pour gels, saving up
to 4 hours preparation time. Stratagene’s quality guarantee means no more gels
lost to bubbles or leaking. All CastAway gels are guaranteed to maintain
stability for a minimum of 60 days from the date of manufacture, with no
detectable deterioration in band resolution.
6% CastAway™ Gels with 32 Preformed Wells
Stratagene adds a new 6% gel to the growing list of precast gels. These new
gels have all the features of the original 6% CastAway gels with the added
benefit of preformed wells. Preformed wells eliminate the need to insert a
sharks-tooth comb into the gel, simplifying set up and sample loading. Preformed
wells also lessen the risk of crossover from neighboring gel lanes, reducing
contamination of adjacent samples. Each well is 3.5-mm wide for easy rinsing and
loading of sample volumes of up to 4.5 µl.
6% Nondenaturing CastAway™ Gels
With the release of the new nondenaturing gels for the CastAway system,
applications requiring native polyacrylamide gels, such as SSCP, can now be run
in a fraction of the time of manually poured gels. The new prepoured
nondenaturing CastAway gels are composed of 6% polyacrylamide in 1X TBE.
Native polyacrylamide gels can be advantageous in applications such as
differential display since higher resolution denaturing polyacrylamide gels
produce data that can be too complex for differential display and SSCP analysis.
For each unique cDNA fragment, nondenaturing gels exhibit a single band where
you would see several bands on a denaturing polyacrylamide gel. This means
that nondenaturing gels are the ideal choice for many applications.
5.5% Long Ranger™ Gels
Stratagene has joined forces with FMC Corporation, manufacturers of the Long
Ranger™ sequencing gel solution, to bring you CastAway™ precast gels made
with the Long Ranger gel matrix. This new CastAway gel is composed of 5.5%
Long Ranger gel solution, 8.3M urea and 1X TBE.
The Long Ranger gel solution is a chemically unique gel formulation,
optimized for manual DNA sequencing. The larger, more uniform pores allow
faster DNA migration than an equal percentage acrylamide gel. The Long Ranger
gels provide 30% more information than polyacrylamide gels and allow you to
either read more sequence or complete short extensions faster. The speed and
convenience of the CastAway system, combined with the longer read capabilities
of the Long Ranger solution, make the Long Ranger gels for the CastAway system
ideal for high-resolution, peak-performance sequencing.
This CastAway gel is made with FMC’s Long Ranger™ sequencing gel
solution, a high-performance gel matrix that delivers superior resolution and
longer read length.
12% CastAway™ Gels with 32 Preformed Wells
Stratagene adds 12% CastAway precast gels with 32 preformed wells to the
CastAway system product line. The new 12% CastAway gels are ideal for DNA
footprinting, analysis of oligonucleotides and other applications requiring the
use of high-percentage polyacrylamide gels for resolution of smaller DNA
fragments. The12% CastAway gels feature 32 preformed wells, which reduce
crossover contamination from adjacent wells, and have a loading volume of up to
4.5 µl. The preformed wells were designed to accommodate 8-channel
pipettors for easy loading of multiple samples.
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